Downregulation of miR-21 Enhances Chemotherapeutic Effect of Taxol in Breast Carcinoma Cells

被引:113
作者
Mei, Mei [5 ]
Ren, Yu [5 ]
Zhou, Xuan [4 ]
Yuan, Xu-bo [3 ]
Han, Lei [1 ]
Wang, Guang-xiu [1 ]
Jia, Zhifan [1 ]
Pu, Pei-yu [1 ]
Kang, Chun-sheng [1 ]
Yao, Zhi [2 ]
机构
[1] Tianjin Med Univ, Gen Hosp, Dept Neurosurg, Tianjin Neurol Inst,Lab Neurooncol, Tianjin 300052, Peoples R China
[2] Tianjin Med Univ, Dept Immunol, Educ Minist, Key Lab Immuno Microenvironm & Dis, Tianjin 300070, Peoples R China
[3] Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300072, Peoples R China
[4] Tianjin Med Univ, Canc Inst & Hosp, Dept Head & Neck Surg 1, Tianjin 300060, Peoples R China
[5] Tianjin Med Univ, Tianjin Res Ctr Basic Med Sci, Tianjin 300070, Peoples R China
关键词
Taxol; MicroRNA-21; PAMAM dendrimer; Breast carcinoma; CANCER; RESISTANCE; EXPRESSION; INDUCTION;
D O I
10.1177/153303461000900109
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The successful of anti-cancer treatment are often limited by the development of drug resistance. Recent work has highlighted the involvement of non-coding RNAs, microRNAs(miRNAs) in cancer development, and their possible involvement in the evolution of drug resistance has been proposed. In this study, we combine taxol chemotherapy and miR-21 inhibitor treatment via polyamidoamine (PAMAM) dendrimers vector to evaluate the effects of combination therapy on suppression of breast cancer cells. The 50% inhibitory concentration (IC50) values for taxol were significantly decreased to a greater extent in the cells transfected with miR-21 inhibitor compared with cells treated with taxol alone. Taxol treatment also increased the percentage of apoptotic breast cancer cells in miR-21 inhibitor transfected cells compared with control cells. Furthermore, treatment of the miR-21 inhibitor-transfected cells with the anti-cancer drugs taxol resulted in significantly reduced cell viability and invasiveness compared with control cells. These results indicated that the miR-21 plays an important role in the resistance of breast carcinoma cells to chemotherapeutic drugs. Therefore, miR-21 inhibitor gene therapy combined with taxol chemotherapy might represent a promising novel therapeutic approach for the treatment of breast malignancies.
引用
收藏
页码:77 / 86
页数:10
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