The Mechanochemistry of Endocytosis

被引:163
作者
Liu, Jian [1 ]
Sun, Yidi [1 ]
Drubin, David G. [1 ]
Oster, George F. [1 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
来源
PLOS BIOLOGY | 2009年 / 7卷 / 09期
基金
美国国家科学基金会;
关键词
CLATHRIN-MEDIATED ENDOCYTOSIS; MEMBRANE CURVATURE; COATED PITS; BAR-DOMAIN; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; ACTIN CYTOSKELETON; VESICLE FORMATION; PLASMA-MEMBRANE; MULTIPLE STAGES; IMAGING ACTIN;
D O I
10.1371/journal.pbio.1000204
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Endocytic vesicle formation is a complex process that couples sequential protein recruitment and lipid modifications with dramatic shape transformations of the plasma membrane. Although individual molecular players have been studied intensively, how they all fit into a coherent picture of endocytosis remains unclear. That is, how the proper temporal and spatial coordination of endocytic events is achieved and what drives vesicle scission are not known. Drawing upon detailed knowledge from experiments in yeast, we develop the first integrated mechanochemical model that quantitatively recapitulates the temporal and spatial progression of endocytic events leading to vesicle scission. The central idea is that membrane curvature is coupled to the accompanying biochemical reactions. This coupling ensures that the process is robust and culminates in an interfacial force that pinches off the vesicle. Calculated phase diagrams reproduce endocytic mutant phenotypes observed in experiments and predict unique testable endocytic phenotypes in yeast and mammalian cells. The combination of experiments and theory in this work suggest a unified mechanism for endocytic vesicle formation across eukaryotes.
引用
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页数:16
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