Effect of Calcium-Modulating Cyclophilin Ligand on Human Immunodeficiency Virus Type 1 Particle Release and Cell Surface Expression of Tetherin

被引：6

作者：

Bego, Mariana G. ^{[1
,2
]}

Dube, Mathieu ^{[1
,2
]}

Mercier, Johanne ^{[1
]}

Cohen, Eric A. ^{[1
,2
]}

机构：

[1] Inst Rech Clin Montreal, Lab Human Retrovirol, Montreal, PQ H2W 1R7, Canada

[2] Univ Montreal, Dept Microbiol & Immunol, Montreal, PQ H3C 3J7, Canada

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 24期

关键词：

VPU PROTEIN; HIV-1; RELEASE; T-CELLS; GENE; CAML; IDENTIFICATION; NFAT1;

D O I：

10.1128/JVI.01786-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The human immunodeficiency virus type 1 (HIV-1) accessory protein Vpu enhances virus particle release by counteracting a host factor that retains virions at the surfaces of infected cells. It was recently demonstrated that cellular protein BST-2/CD317/Tetherin restricts HIV-1 release in a Vpu-dependent manner. Calcium-modulating cyclophilin ligand (CAML) was also proposed to be involved in this process. We investigated whether CAML is involved in cell surface expression of Tetherin. Here, we show that CAML overexpression in permissive Cos-7 cells or CAML depletion in restrictive HeLa cells has no effect on HIV-1 release or on Tetherin surface expression, indicating that CAML is not required for Tetherin-mediated restriction of HIV-1 release.

引用

页码：13032 / 13036

页数：5

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