Reconstitution, activities, and structure of the eukaryotic RNA exosome

被引:416
作者
Liu, Quansheng
Greimann, Jaclyn C.
Lima, Christopher D. [1 ]
机构
[1] Sloan Kettering Inst, Struct Biol Program, New York, NY 10021 USA
[2] Sloan Kettering Inst, Training Program Chem Biol, New York, NY 10021 USA
基金
美国国家卫生研究院;
关键词
MESSENGER-RNA; POLY(A) POLYMERASE; PROTEIN; DEGRADATION; COMPLEX; COMPONENTS; MECHANISM; SUBUNITS;
D O I
10.1016/j.cell.2006.10.037
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The RNA exosome is a multisubunit 3' to 5' exoribonuclease complex that participates in degradation and processing of cellular RNA. To determine the activities and structure of the eukaryotic exosome, we report the reconstitution of 9-subunit exosomes from yeast and human and reconstitution of 10- and 11-subunit exosomes from yeast. Comparative biochemical analysis between purified subunits and reconstituted exosomes using AU-rich, polyadenylated (poly[A]), generic, and structured RNA substrates reveals processive phosphorolytic activities for human Rrp41/Rrp45 and the 9-subunit human exosome, processive hydrolytic activities for yeast Rrp44 and the yeast 10-sub-unit exosome, distributive hydrolytic activities for Rrp6, and processive and distributive hydrolytic activities for the yeast 11-subunit exosome. To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 angstrom.
引用
收藏
页码:1223 / 1237
页数:15
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