Three-dimensional Ca2+ imaging advances understanding of astrocyte biology

被引：239

作者：

Bindocci, Erika ^{[1
]}

Savtchouk, Iaroslav ^{[1
]}

Liaudet, Nicolas ^{[1
]}

Becker, Denise ^{[1
]}

Carriero, Giovanni ^{[1
]}

Volterra, Andrea ^{[1
]}

机构：

[1] Univ Lausanne, Dept Fundamental Neurosci, CH-1005 Lausanne, Switzerland

来源：

SCIENCE | 2017年 / 356卷 / 6339期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

RESPONSES; DYNAMICS; CORTEX; VASODILATION; MODULATION; ACTIVATION; TRANSIENT; RELEASE; SIGNALS; CELLS;

D O I：

10.1126/science.aai8185

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Astrocyte communication is typically studied by two-dimensional calcium ion (Ca2+) imaging, but this method has not yielded conclusive data on the role of astrocytes in synaptic and vascular function. We developed a three-dimensional two-photon imaging approach and studied Ca2+ dynamics in entire astrocyte volumes, including during axon-astrocyte interactions. In both awake mice and brain slices, we found that Ca2+ activity in an individual astrocyte is scattered throughout the cell, largely compartmented between regions, preponderantly local within regions, and heterogeneously distributed regionally and locally. Processes and endfeet displayed frequent fast activity, whereas the soma was infrequently active. In awake mice, activity was higher than in brain slices, particularly in endfeet and processes, and displayed occasional multifocal cellwide events. Astrocytes responded locally to minimal axonal firing with time-correlated Ca2+ spots.

引用

页数：11

共 42 条

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