Sildenafil relaxes rabbit clitoral corpus cavernosum

被引：32

作者：

Vemulapalli, S ^{[1
]}

Kurowski, S ^{[1
]}

机构：

[1] Schering Plough Res Inst, CNS, CV Biol Res, Kenilworth, NJ 07033 USA

来源：

LIFE SCIENCES | 2000年 / 67卷 / 01期

关键词：

sildenafil; clitoris; L-NAME;

D O I：

10.1016/S0024-3205(00)00596-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The role of phosphodiesterase type 5 inhibition in the modulation of female sexual dysfunction was investigated by assessing its effects on in vitro relaxation of rabbit clitoris. Stimulation of the nonadrenergic, non-cholinergic neurons of the clitorus elicited a frequency-dependent relaxation response. inhibition of NO synthase with L-NAME (100 mu M) or inhibition of soluble guanylate cyclase with ODQ (1.0 mu M) almost completely abolished the electrical field stimulation-induced relaxation of clitorus suggesting that NO-cGMP pathway mediates the relaxation response to electrical field stimulation. Similarly, tetrodotoxin, a neuronal sodium channel blocker abolished the electrical field stimulation-induced clitoral relaxation implying a neuronal release of NO contributes to the electrical field stimulation elicited relaxation. Pretreatment of the clitoral corpus cavernosum strips with sildenafil (100 nM) enhanced the electrical field stimulation-induced relaxations both in magnitude and duration. The results suggest that sildenafil enhances electrical field stimulation elicited clitoral relaxation by a NO-cGMP dependent pathway. These data also imply that sildenafil may be useful to treat female sexual dysfunction, (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：23 / 29

页数：7

共 17 条

[11] Treatment of erectile dysfunction with sildenafil [J].

Marks, LS ;

Duda, C ;

Dorey, FJ ;

Macairan, ML ;

Santos, PB .

UROLOGY, 1999, 53 (01) :19-24

[12] Sildenafil, a novel inhibitor of phosphodiesterase type 5 in human corpus cavernosum smooth muscle cells [J].

Moreland, RB ;

Goldstein, I ;

Traish, A .

LIFE SCIENCES, 1998, 62 (20) :PL309-PL318

[13] Sildenafil inhibits phosphodiesterase type 5 in human clitoral corpus cavernosum smooth muscle [J].

Park, K ;

Moreland, RB ;

Goldstein, I ;

Atala, A ;

Traish, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 249 (03) :612-617

[14] NITRIC-OXIDE AS A MEDIATOR OF RELAXATION OF THE CORPUS CAVERNOSUM IN RESPONSE TO NONADRENERGIC, NONCHOLINERGIC NEUROTRANSMISSION [J].

RAJFER, J ;

ARONSON, WJ ;

BUSH, PA ;

DOREY, FJ ;

IGNARRO, LJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (02) :90-94

[15]

Rand M. J., 1995, P227, DOI 10.1016/B978-012721985-1/50014-9

[16]

Shen WW, 1999, J REPROD MED, V44, P535

[17] Sildenafil (VIAGRA(TM)), a potent and selective inhibitor of type 5 cGMP phosphodiesterase with utility for the treatment of male erectile dysfunction [J].

Terrett, NK ;

Bell, AS ;

Brown, D ;

Ellis, P .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1996, 6 (15) :1819-1824

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