Involvement of protein kinase Cδ in induction of apoptosis by cationic liposomes in macrophage-like RAW264.7 cells

被引：26

作者：

Arisaka, Masaya ^{[1
]}

Nakamura, Tomoko ^{[1
]}

Yamada, Akiko ^{[1
]}

Negishi, Yoichi ^{[1
]}

Aramaki, Yukihiko ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Tokyo 1920392, Japan

来源：

FEBS LETTERS | 2010年 / 584卷 / 05期

关键词：

Apoptosis; Cationic liposome; Macrophage; PKC delta; Reactive oxygen species; PKC-DELTA; ACTIVATION; PROTEOGLYCANS; DELIVERY; RELEASE;

D O I：

10.1016/j.febslet.2010.01.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently demonstrated that reactive oxygen species (ROS) play an important role in RAW264.7 cell apoptosis induced by cationic liposomes composed of stearylamine (SA-liposomes). In this study, we investigated whether protein kinase C delta PKC delta) is involved in apoptosis induced by cationic liposomes. Tyrosine phosphorylation, nuclear localization, and cleavage of PKCd were observed following the treatment of cells with SA-liposomes, suggesting that SA-liposomes activate PKCd. Rottlerin, a specific inhibitor of PKCd, inhibited ROS generation and also suppressed apoptosis. Cell surface proteoglycans may contribute to PKCd activation by SA-liposomes. These findings suggest that PKCd is strongly associated with apoptosis induced by SA-liposomes. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1016 / 1020

页数：5

共 25 条

[1] Drug delivery systems: Entering the mainstream [J].