Probing the Activity of Matrix Metalloproteinase II with a Sequentially Click-Labeled Silica Nanoparticle FRET Probe

被引：30

作者：

Achatz, Daniela E. ^{[3
]}

Mezo, Gabor ^{[2
]}

Kele, Peter ^{[1
]}

Wolfbeis, Otto S. ^{[3
]}

机构：

[1] Eotvos Lorand Univ, Inst Chem, Pazmany Peter Setany 1A, H-1117 Budapest, Hungary

[2] Eotvos Lorand Univ, Hungarian Acad Sci, Res Grp Peptide Chem, H-1117 Budapest, Hungary

[3] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93040 Regensburg, Germany

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 14期

关键词：

click chemistry; fluorescence; FRET; MMP-2; nanoparticles; silica; PROTEASE ACTIVITY; QUANTUM DOTS; LIVING CELLS; COPPER-FREE; FLUORESCENT; CHEMISTRY; CYCLOADDITION; LIGATION; SURFACES;

D O I：

10.1002/cbic.200900261

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) Selective double click labeling: The determination of nanomolar concentrations of matrix metalloproteinase II is made possible through the use of a nanoprobe that consists of fluorescently doped silica nanoparticles to which a FRET-based enzyme substrate has been conjugated through sequential click reactions. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2316 / 2320

页数：5

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