Defensive strategies against high light stress in wild and D1 protein mutant biotypes of Erigeron canadensis

被引:11
作者
Darkó, É
Váradi, G
Lemoine, Y
Lehoczki, E [1 ]
机构
[1] Attila Jozsef Univ, Dept Bot, H-6701 Szeged, Hungary
[2] Univ Lille 1, CNRS, UPRES A 8013, F-59655 Villeneuve Dascq, France
[3] Res Inst Viticulture & Enol, H-6000 Kecskemet, Hungary
来源
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY | 2000年 / 27卷 / 04期
关键词
chlorophyll fluorescence; Erigeron canadensis; herbicide resistance; high light stress; xanthophyll cycle;
D O I
10.1071/PP99097
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The Ser(264)-->Gly substitution on the D1 protein is accompanied by a higher photosensitivity of the mutant plant. This may be due to an increased D1 protein turnover and/or to a lower xanthophyll cycle activity in vivo. The relative importance of these two photoprotective mechanisms in wild and D1 protein mutant biotypes of Erigeron canadensis L. was established by using dithiothreitol and streptomycin. Moreover, the interconversion of violaxanthin to zeaxanthin via antheraxanthin was studied in isolated thylakoids and in intact leaves treated with paraquat. Streptomycin caused a more severe decrease in the optimal quantum yield (F-v/F-m) of PS II and a large increase in the initial fluorescence yield (F-o) in the mutant compared to the wild biotype. In the fluorescence-quenching parameters of the wild-type leaves, dithiothreitol caused alterations similar to those observed in the mutant plant without dithiothreitol. A lowered activity of the xanthophyll cycle was detected in the mutant biotype compared to the wild-type in vivo. However, under in vitro, conditions which were optimal for violaxanthin de-epoxidation, or when paraquat was used on intact leaves to accelerate the electron transport, violaxanthin could readily be converted to zeaxanthin even in the mutant plants. This demonstrates that neither the decrease in the enzymatic activity of violaxanthin de-epoxidase nor the low availability of violaxanthin is responsible for the low zeaxanthin formation under in vivo conditions. It is presumed that, in vivo, the D1 protein mutation results in slower electron transport, a smaller Delta pH and lower zeaxanthin formation, and thereby in alterations in the defensive strategies against high light illumination.
引用
收藏
页码:325 / 333
页数:9
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