Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKTI1

被引:301
作者
Mäser, P
Eckelman, B
Vaidyanathan, R
Horie, T
Fairbairn, DJ
Kubo, M
Yamagami, M
Yamaguchi, K
Nishimura, M
Uozumi, N
Robertson, W
Sussman, MR
Schroeder, JI
机构
[1] Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Cell & Dev Biol Sect, La Jolla, CA 92093 USA
[3] Univ Queensland, Dept Bot, St Lucia, Qld 4072, Australia
[4] Nagoya Univ, Biosci Ctr, Nagoya, Aichi 4648601, Japan
[5] Inst Environm Sci, Aomori 0393212, Japan
[6] Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan
[7] Univ Wisconsin, Ctr Biotechnol, Madison, WI 53706 USA
关键词
sodium transport; salt stress; T-DNA insertion; Arabidopsis thaliana;
D O I
10.1016/S0014-5793(02)03488-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sodium (Na+) is toxic to most plants, but the molecular mechanisms of plant Na+ uptake and distribution remain largely unknown. Here we analyze Arabidopsis lines disrupted in the Na+ transporter AtHKT1. AtHKT1 is expressed in the root stele and leaf vasculature. athkt1 null plants exhibit lower root Na+ levels and are more salt resistant than wild-type in short-term root growth assays. In shoot tissues, however, athkt1 disruption produces higher Na+ levels, and athkt1 and athktl/sos3 shoots are Na+-hypersensitive in long-term growth assays. Thus wild-type AtHKT1 controls root/shoot Na+ distribution and counteracts salt stress in leaves by reducing leaf Na+ accumulation. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:157 / 161
页数:5
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