Recovery of replication-competent HIV despite prolonged suppression of plasma viremia

被引：1845

作者：

Wong, JK

Hezareh, M

Gunthard, HF

Havlir, DV

Ignacio, CC

Spina, CA

Richman, DD

机构：

[1] UNIV CALIF SAN DIEGO,SCH MED,DEPT PATHOL,LA JOLLA,CA 92093

[2] UNIV CALIF SAN DIEGO,TREATMENT CTR,SAN DIEGO,CA 92103

[3] VET AFFAIRS SAN DIEGO HEALTHCARE SYST,SAN DIEGO,CA 92063

来源：

SCIENCE | 1997年 / 278卷 / 5341期

关键词：

D O I：

10.1126/science.278.5341.1291

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In evaluating current combination drug regimens for treatment of human immunodeficiency virus (HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8 cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors, activation of patient CD4 lymphocytes with immobilized antibodies to CD3 and CD28 enabled the isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to fewer than 50 copies per milliliter for up to 2 years. Partial sequencing of HIV pol revealed no new drug resistance mutations or discernible evolution, providing evidence for viral latency rather than drug failure.

引用

页码：1291 / 1295

页数：5

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