The stability of cortical microtubules depends on their orientation

被引:49
作者
Wiesler, B [1 ]
Wang, QY [1 ]
Nick, P [1 ]
机构
[1] Univ Freiburg, Inst Biol Bot 2, D-79104 Freiburg, Germany
关键词
auxin; de-tyrosination; maize; microtubules; post-translational modification; tubulin;
D O I
10.1046/j.1365-313X.2002.01489.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Auxin controls the orientation of cortical microtubules in maize coleoptile segments. We used tyrosinylated alpha-tubulin as a marker to assess auxin-dependent changes in microtubule turnover. Auxin-induced tyrosinylated alpha-tubulin, correlated with an elevated sensitivity of growth to antimicrotubular compounds such as ethyl-N -phenylcarbamate (EPC). We determined the affinity of alpha-tubulin to EPC and found that it was dramatically increased when the tubulin was de-tyrosinylated. By proteolytic cleavage of the carboxy terminal tyrosine, such an increased affinity could be induced in vitro . Thus, the auxin-induced sensitivity of growth to EPC is not caused by an increased affinity for this inhibitor, but caused by a reduced microtubule turnover. Double visualization assays revealed that the transverse microtubules induced by auxin consist predominantly of tyrosinylated alpha-tubulin, whereas the longitudinal microtubules induced by auxin depletion contain de-tyrosinylated alpha-tubulin. The results are discussed in terms of direction-dependent differences in the lifetime of microtubules.
引用
收藏
页码:1023 / 1032
页数:10
相关论文
共 29 条
[11]   TUBULIN GENE-EXPRESSION IN MAIZE (ZEA-MAYS L) - CHANGE IN ISOTYPE EXPRESSION ALONG THE DEVELOPMENTAL AXIS OF SEEDLING ROOT [J].
JOYCE, CM ;
VILLEMUR, R ;
SNUSTAD, DP ;
SILFLOW, CD .
JOURNAL OF MOLECULAR BIOLOGY, 1992, 227 (01) :97-107
[12]   ENHANCED STABILITY OF MICROTUBULES ENRICHED IN DETYROSINATED TUBULIN IS NOT A DIRECT FUNCTION OF DETYROSINATION LEVEL [J].
KHAWAJA, S ;
GUNDERSEN, GG ;
BULINSKI, JC .
JOURNAL OF CELL BIOLOGY, 1988, 106 (01) :141-149
[13]   RAT MONOCLONAL ANTITUBULIN ANTIBODIES DERIVED BY USING A NEW NON-SECRETING RAT-CELL LINE [J].
KILMARTIN, JV ;
WRIGHT, B ;
MILSTEIN, C .
JOURNAL OF CELL BIOLOGY, 1982, 93 (03) :576-582
[14]   MICROTUBULES CONTAINING DETYROSINATED TUBULIN ARE LESS DYNAMIC [J].
KREIS, TE .
EMBO JOURNAL, 1987, 6 (09) :2597-2606
[15]  
KUMAR N, 1981, J BIOL CHEM, V256, P7678
[16]   WHY SHOULD STATIONARY PLANT-CELLS HAVE SUCH DYNAMIC MICROTUBULES [J].
LLOYD, C .
MOLECULAR BIOLOGY OF THE CELL, 1994, 5 (12) :1277-1280
[17]   A NEW SPRING FOR PLANT-CELL BIOLOGY - MICROTUBULES AS DYNAMIC HELICES [J].
LLOYD, CW ;
SEAGULL, RW .
TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (12) :476-478
[18]   Tubulin post-translational modifications - Enzymes and their mechanisms of action [J].
MacRae, TH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 244 (02) :265-278
[19]   EFFECTS OF ANTI-MICROTUBULE DRUGS ON INVITRO POLYMERIZATION OF TUBULIN FROM MUNG BEAN [J].
MIZUNO, K ;
SUZAKI, T .
BOTANICAL MAGAZINE-TOKYO, 1990, 103 (1072) :435-448
[20]   ISOLATION OF PLANT TUBULIN FROM AZUKI BEAN EPICOTYLS BY ETHYL N-PHENYLCARBAMATE-SEPHAROSE AFFINITY-CHROMATOGRAPHY [J].
MIZUNO, K ;
KOYAMA, M ;
SHIBAOKA, H .
JOURNAL OF BIOCHEMISTRY, 1981, 89 (01) :329-332