Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum

被引:42
作者
Meyerholz, DK
Stabel, TJ
Ackermann, MR
Carlson, SA
Jones, BD
Pohlenz, J
机构
[1] USDA ARS, Natl Anim Dis Ctr, Preharvest Food Safety & Enter Dis Unit, Ames, IA 50010 USA
[2] Iowa State Univ, Dept Vet Microbiol, Ames, IA USA
[3] Iowa State Univ, Dept Vet Pathol, Ames, IA USA
[4] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA
[5] Tierarztlichen Hsch Hannover, Inst Pathol, D-3000 Hannover, Germany
关键词
DT104; electron microscopy; enterocyte; extrusion; ileum; invasion; Salmonella; swine; Typhimurium;
D O I
10.1354/vp.39-6-712
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Salmonella enterica serovar Typhimurium is an important intestinal pathogen in swine. This study was performed to document the early cellular invasion of Salmonella serovar Typhimurium in swine ileum. Ileal gut-loops were surgically prepared in ten 4- to 5-week-old mixed-breed pigs and inoculated for 0-60 minutes. Loops were harvested and prepared for both scanning and transmission electron microscopy (SEM and TEM, respectively). Preferential bacterial adherence to microfold cells (M cells) was seen within 5 minutes, and by 10 minutes bacterial invasion of the apical membrane was seen in M cells, goblet cells, and enterocytes. This multicellular invasion was observed throughout the course of infection. In addition, SEM revealed a specific affinity of Salmonella serovar Typhimurium to sites of cell extrusion. Using TEM, bacteria in these areas were focused in the crevices formed by the extruding cell and the adjacent cells and in the cytoplasm immediately beneath the extruding cell. Our results suggest that early cellular invasion by Salmonella serovar Typhimurium is nonspecific and rapid in swine. Furthermore, the combination of SEM and TEM data suggests that Salmonella serovar Typhimurium may use sites of cell extrusion as an additional mechanism for early invasion.
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页码:712 / 720
页数:9
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[31]   The isoflavone genistein inhibits internalization of enteric bacteria by cultured Caco-2 and HT-29 enterocytes [J].
Wells, CL ;
Jechorek, RP ;
Kinneberg, KM ;
Debol, SM ;
Erlandsen, SL .
JOURNAL OF NUTRITION, 1999, 129 (03) :634-640