Coupling conducting polymers and mediated electrochemical responses for the detection of Listeria

被引:19
作者
Minett, AI [1 ]
Barisci, JN [1 ]
Wallace, GG [1 ]
机构
[1] Univ Wollongong, Intelligent Polymer Res Inst, Wollongong, NSW 2522, Australia
基金
澳大利亚研究理事会;
关键词
Listeria; biosensor; signal generation techniques;
D O I
10.1016/S0003-2670(02)01033-4
中图分类号
O65 [分析化学];
学科分类号
070302 [分析化学]; 081704 [应用化学];
摘要
Different signal generation techniques were investigated for the development of a biosensor for Listeria monocytogenes. Conventional amperometry at an antibody-containing polypyrrole film electrode was found to be unsuccessful in detecting levels below 10(6) cells ml(-1). More successful was the coupling of a covalently modified film with the use of electron mediators in a single device. This sensor was capable of reproducibly detecting Listeria at levels of 10(5) cells ml(-1) in 30 min. (C) 2002 Elsevier Science B.V All rights reserved.
引用
收藏
页码:37 / 45
页数:9
相关论文
共 27 条
[1]
Highly sensitive flow-injection immunoassay system for rapid detection of bacteria [J].
Abdel-Hamid, I ;
Ivnitski, D ;
Atanasov, P ;
Wilkins, E .
ANALYTICA CHIMICA ACTA, 1999, 399 (1-2) :99-108
[2]
Abdel-Hamid I, 1998, ELECTROANAL, V10, P758, DOI 10.1002/(SICI)1521-4109(199809)10:11<758::AID-ELAN758>3.0.CO
[3]
2-5
[4]
Electroimmobilisation of sulphite oxidase into a polypyrrole film and its utilisation for flow amperometric detection of sulphite [J].
Adeloju, SB ;
Barisci, JN ;
Wallace, GG .
ANALYTICA CHIMICA ACTA, 1996, 332 (2-3) :145-153
[5]
ALVAREZICAZA M, 1993, ANAL CHEM, V65, P525
[6]
BARTLETT P N, 1991, Progress in Reaction Kinetics, V16, P55
[7]
BUSH DL, 1987, ANAL LETT, V20, P1781
[8]
ELECTROCATALYTIC OXIDATION OF REDUCED NICOTINAMIDE COENZYMES AT ORGANIC DYE-MODIFIED ELECTRODES [J].
CHI, Q ;
DONG, S .
ELECTROANALYSIS, 1995, 7 (02) :147-153
[9]
Clark W.M., 1960, OXIDATION REDUCTION
[10]
Guilbault George G., 1994, V60, P151