A role for TFIIIC transcription factor complex in genome organization

被引:237
作者
Noma, Ken-ichi
Cam, Hugh P.
Maraia, Richard J.
Grewal, Shiv I. S. [1 ]
机构
[1] NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA
[2] NICHHD, Lab Mol Growth Regulat, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1016/j.cell.2006.04.028
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eukaryotic genome complexity necessitates boundary and insulator elements to partition genomic content into distinct domains. We show that inverted repeat (IR) boundary elements flanking the fission yeast mating-type heterochromatin domain contain B-box sequences, which prevent heterochromatin from spreading into neighboring euchromatic regions by recruiting transcription factor TFIIIC complex without RNA polymerase III (Pol III). Genome-wide analysis reveals TFIIIC with Pol III at all tRNA genes, many of which cluster at pericentromeric heterochromatin domain boundaries. However, a single tRNA(phe), gene with modest TFIIIC enrichment is insufficient to serve as boundary and requires RNAi-associated element to restrain heterochromatin spreading. Remarkably, we found TFIIIC localization without Pol III at many sites located between divergent promoters. These sites appear to act as chromosome-organizing clamps by tethering distant loci to the nuclear periphery, at which TFIIIC: is concentrated into several distinct bodies. Our analyses uncover a general genome organization mechanism involving conserved TFIIIC complex.
引用
收藏
页码:859 / 872
页数:14
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