Differential inhibition of telomerase activity during induction of differentiation in hematopoietic, melanoma, and glioma cells in culture

被引:16
作者
Cheng, AJ
Liao, SK
Chow, SE
Chen, JK
Wang, TCV
机构
[1] CHANG GUNG COLL MED & TECHNOL,DEPT BIOL MOL,TAYUAN 333,TAIWAN
[2] CHANG GUNG COLL MED & TECHNOL,GRAD INST CLIN MED,TAYUAN 333,TAIWAN
[3] CHANG GUNG COLL MED & TECHNOL,DEPT PHYSIOL,TAYUAN 333,TAIWAN
关键词
D O I
10.1006/bbrc.1997.7157
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Accumulating evidence indicates that telomerase activity is stringently repressed in normal human somatic cells but reactivated in cancers and immortal cells, suggesting that activation of telomerase activity may play a role in carcinogenesis and immortalization. Recently, down-regulation of telomerase activity by induction of differentiation has been reported for cells of pre-myelocytic and myelocytic leukemia as web as embryonic carcinoma. To gain further insight about the regulation of telomerase activity following induction of differentiation, telomerase activity was examined in a human hematopoietic progenitor cell line (D2), a melanoma cell line (CM73-36) and a glioma cell line (Ast812) before and after addition of differentiation inducing agents. The state of differentiation was assessed by growth inhibition and cell morphological maturation. Telomerase activity was assayed by a PCR-based telomeric repeat amplification protocol (TRAP). Our data show that telomerase activity was inhibited only in differentiation-induced D2 cells but not in differentiation-induced melanoma and glioma Ils. A model for the differential inhibition of telomerase activity following induction of differentiation in different cancer cells will be presented. (C) 1997 Academic Press.
引用
收藏
页码:438 / 444
页数:7
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