Impedimetric immunosensor using avidin-biotin for antibody immobilization

被引：79

作者：

Ouerghi, O

Touhami, A

Jaffrezic-Renault, N

Martelet, C

Ben Ouada, H

Cosnier, S

机构：

[1] Ecole Cent Lyon, CNRS, UMR 5621, F-69131 Ecully, France

[2] Fac Sci Monastir, Lab Phys Interfaces, Monastir, Tunisia

[3] Univ Grenoble 1, CNRS, UMR 5630, Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble, France

来源：

BIOELECTROCHEMISTRY | 2002年 / 56卷 / 1-2期

关键词：

electropolymerization; biotinylated polypyrrole; impedance spectroscopy; immunosensor;

D O I：

10.1016/S1567-5394(02)00029-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The potentialities of an electrodeposited biotinylated polypyrrole film as an immobilisation matrix for the fabrication of impedimetric immunosensors are described. Biotinylated antibody (anti-human IgG), used as a model system, was attached to free biotin groups on the electrogenerated. polypyrrole film using avidin as a coupling reagent. This immobilization method allows to obtain a highly reproducible and stable device. The resulting immunosensor has a linear dynamic range of 10-80 ng ml(-1) of antigen and a detection limit of 10 pg ml(-1). Furthermore, this immunosensor exhibited minor loss in response after two regeneration steps. (C) 2002 Elsevier Science B.V All rights reserved.

引用

页码：131 / 133

页数：3

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