A cryptic DNA binding domain at the COOH terminus of TFIIIB70 affects formation, stability, and function of preinitiation complexes

被引：26

作者：

Huet, J ^{[1
]}

Conesa, C ^{[1
]}

Carles, C ^{[1
]}

Sentenac, A ^{[1
]}

机构：

[1] CEA SACLAY,SERV BIOCHIM & GENET MOL,F-91191 GIF SUR YVETTE,FRANCE

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1997年 / 272卷 / 29期

关键词：

D O I：

10.1074/jbc.272.29.18341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TFIIIC-dependent assembly of yeast TFIIIB on class III genes unmasks a high avidity of TFIIIB for DNA. TFIIIB contains TATA-binding protein (TBP), TFIIIB90/B '', and TFIIIB70/Brf1, which is homologous to TFIIB. Using limited proteolysis, we have found that the COOH terminus of TFIIIB70 (residues 510-596) forms a protease-resistant domain that binds DNA tightly as seen by Southwestern, DNase I footprinting, and gel shift assays. Consistent with a role for this DNA binding activity, preinitiation complexes were formed less efficiently with truncated TFIIIB70 lacking the COOH-terminal domain and displayed an increased sensitivity to heparin, B' (TFIIIB70 + TBP).TFIIIC.DNA complexes were also particularly unstable, In addition, TFIIIB.TFIIIC.DNA complexes containing truncated TFIIIB70 were im paired in promoting transcription initiation.

引用

页码：18341 / 18349

页数：9

共 61 条

[1] STIMULATION OF TRANSCRIPT ELONGATION REQUIRES BOTH THE ZINC FINGER AND RNA POLYMERASE-II BINDING DOMAINS OF HUMAN TFIIS [J].

AGARWAL, K ;

BAEK, KH ;

JEON, CJ ;

MIYAMOTO, K ;

UENO, A ;

YOON, HS .

BIOCHEMISTRY, 1991, 30 (31) :7842-7851

[2] SOLUTION STRUCTURE OF THE C-TERMINAL CORE DOMAIN OF HUMAN TFIIB - SIMILARITY TO CYCLIN-A AND INTERACTION WITH TATA-BINDING PROTEIN [J].

BAGBY, S ;

KIM, SJ ;

MALDONADO, E ;

TONG, KI ;

REINBERG, D ;

IKURA, M .

CELL, 1995, 82 (05) :857-867

[3] NUCLEOTIDE-SEQUENCES OF YEAST GENES FOR TRANSFER-RNA 2SER, TRANSFER-RNA 1VAL - HOMOLOGY BLOCKS OCCUR IN THE VICINITY OF DIFFERENT TRANSFER-RNA GENES [J].

BAKER, RE ;

EIGEL, A ;

VOGTEL, D ;

FELDMANN, H .

EMBO JOURNAL, 1982, 1 (03) :291-295

[4] STRUCTURAL FEATURES OF YEAST TRANSFER-RNA GENES WHICH AFFECT TRANSCRIPTION FACTOR BINDING [J].

BAKER, RE ;

HALL, BD .

EMBO JOURNAL, 1984, 3 (12) :2793-2800

[5] DELINEATION OF 2 FUNCTIONAL REGIONS OF TRANSCRIPTION FACTOR-TFIIB [J].

BARBERIS, A ;

MULLER, CW ;

HARRISON, SC ;

PTASHNE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5628-5632

[6] 2 COMPONENTS OF SACCHAROMYCES-CEREVISIAE TRANSCRIPTION FACTOR-IIIB (TFIIIB) ARE STEREOSPECIFICALLY LOCATED UPSTREAM OF A TRANSFER-RNA GENE AND INTERACT WITH THE 2ND-LARGEST SUBUNIT OF TFIIIC [J].

BARTHOLOMEW, B ;

KASSAVETIS, GA ;

GEIDUSCHEK, EP .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (10) :5181-5189

[7]

BRAUN BR, 1992, J BIOL CHEM, V267, P22562

[8] A SUPPRESSOR OF TBP MUTATIONS ENCODES AN RNA POLYMERASE-III TRANSCRIPTION FACTOR WITH HOMOLOGY TO TFIIB [J].

BURATOWSKI, S ;

ZHOU, H .

CELL, 1992, 71 (02) :221-230

[9] FUNCTIONAL DOMAINS OF TRANSCRIPTION FACTOR-TFIIB [J].

BURATOWSKI, S ;

ZHOU, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5633-5637

[10] TFIIIC RELIEVES REPRESSION OF U6 SNRNA TRANSCRIPTION BY CHROMATIN [J].

BURNOL, AF ;

MARGOTTIN, F ;

HUET, J ;

ALMOUZNI, G ;

PRIOLEAU, MN ;

MECHALI, M ;

SENTENAC, A .

NATURE, 1993, 362 (6419) :475-477

← 1 2 3 4 5 6 7 →