"The heat is on": Rapid microcalorimetric detection of mycobacteria in culture

被引：33

作者：

Braissant, O. ^{[1
]}

Wirz, D. ^{[1
]}

Goepfert, B. ^{[1
]}

Daniels, A. U. ^{[1
]}

机构：

[1] Univ Basel, Lab Biomech & Biocalorimetry LOB2, Fac Med, Biozentrum Pharmazentrum, CH-4056 Basel, Switzerland

来源：

TUBERCULOSIS | 2010年 / 90卷 / 01期

关键词：

Mycobacterium tuberculosis; Mycobacteria; Microcalorimetry; Detection; STAPHYLOCOCCUS-AUREUS; THERMOPILE;

D O I：

10.1016/j.tube.2009.11.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Detection of mycobacterial infection can be achieved by different means; however, culture-based methods remain the gold standard. In this paper, we present a new culture-based method using real-time microcalorimetric detection of growth of Mycobacterium species including Mycobacterium tuberculosis. Microcalorimetric detection of heat production by 6 different growing species of Mycobacterium was achieved between 20 and 310 h depending on their type (fast vs. slow-growing mycobacteria) and initial concentration. This study demonstrates that microcalorimetric detection of mycobacterial growth is a potential advantageous alternative to methods using fluorescent or radiolabeled growth indicators. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：57 / 59

页数：3

共 15 条

[1]

[Anonymous], 2008, NEW LAB DIAGN TOOLS

[2]

Performance of Microcalorimetry for Early Detection of Methicillin Resistance in Clinical Isolates of Staphylococcus aureus [J].