Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice

被引：253

作者：

Ohta, M

Hayashi, Y

Nakashima, A

Hamada, A

Tanaka, A

Nakamura, T

Hayakawa, T

机构：

[1] Plantech Res Inst, Aoba Ku, Yokohama, Kanagawa 2270033, Japan

[2] Chiba Univ, Fac Pharmaceut Sci, Mol Cell Biol Lab, Chiba 2638522, Japan

来源：

FEBS LETTERS | 2002年 / 532卷 / 03期

关键词：

halophyte plants; sodium/proton antiporter; rice; salt tolerance; transgenic plants;

D O I：

10.1016/S0014-5793(02)03679-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by nonstress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：279 / 282

页数：4

相关论文

共 29 条

[21] FERTILE TRANSGENIC RICE PLANTS REGENERATED FROM TRANSFORMED PROTOPLASTS [J].

SHIMAMOTO, K ;

TERADA, R ;

IZAWA, T ;

FUJIMOTO, H .

NATURE, 1989, 338 (6212) :274-276

[22] NA+/H+ ANTIPORT ACTIVITY IN TONOPLAST VESICLES FROM ROOTS OF THE SALT-TOLERANT PLANTAGO-MARITIMA AND THE SALT-SENSITIVE PLANTAGO-MEDIA [J].

STAAL, M ;

MAATHUIS, FJM ;

ELZENGA, JTM ;

OVERBEEK, JHM ;

PRINS, HBA .

PHYSIOLOGIA PLANTARUM, 1991, 82 (02) :179-184

[23] H+-TRANSLOCATING ATPASES - ADVANCES USING MEMBRANE-VESICLES [J].

SZE, H .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1985, 36 :175-208

[24] ENHANCEMENT OF FOREIGN GENE-EXPRESSION BY A DICOT INTRON IN RICE BUT NOT IN TOBACCO IS CORRELATED WITH AN INCREASED LEVEL OF MESSENGER-RNA AND AN EFFICIENT SPLICING OF THE INTRON [J].

TANAKA, A ;

MITA, S ;

OHTA, S ;

KYOZUKA, J ;

SHIMAMOTO, K ;

NAKAMURA, K .

NUCLEIC ACIDS RESEARCH, 1990, 18 (23) :6767-6770

[25] Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice [J].

Xu, DP ;

Duan, XL ;

Wang, BY ;

Hong, BM ;

Ho, THD ;

Wu, R .

PLANT PHYSIOLOGY, 1996, 110 (01) :249-257

[26] BINDING OF 7-CHLORO-4-NITROBENZO-2-OXA-1,3-DIAZOLE TO AN ESSENTIAL CYSTEINE RESIDUE(S) IN THE TONOPLAST H+-ATPASE FROM MUNG BEAN (VIGNA-RADIATA L) HYPOCOTYLS [J].

YAMANISHI, H ;

KASAMO, K .

PLANT PHYSIOLOGY, 1992, 99 (02) :652-658

[27]

Yoshida S., 1976, LAB MANUAL PHYSL STU

[28] Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit [J].

Zhang, HX ;

Blumwald, E .

NATURE BIOTECHNOLOGY, 2001, 19 (08) :765-768

[29] Engineering salt-tolerant Brassica plants:: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation [J].

Zhang, HX ;

Hodson, JN ;

Williams, JP ;

Blumwald, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (22) :12832-12836