Solid-phase synthesis of CD52 glycopeptide and an efficient route to asn-core pentasaccharide conjugate

被引：32

作者：

Guo, ZW ^{[1
]}

Nakahara, Y ^{[1
]}

Ogawa, T ^{[1
]}

机构：

[1] UNIV TOKYO,GRAD SCH AGR & LIFE SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1997年 / 5卷 / 10期

关键词：

D O I：

10.1016/S0968-0896(97)00126-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intact peptide sequence (18) as well as its glycoform carrying an N-linked core pentasaccharide (I) of CD52 antigen were prepared by means of solid-phase synthesis employing Fmoc-amino acids and benzyl-protected oligo saccharide-asparagine conjugate (3) as building blocks. It was concluded that the pentasaccharide structure had little influence on further peptide elongation in solid-phase synthesis and the benzylated pentasaccharide moiety was sufficiently stable to the 95% TFA acidic conditions used to release glycopeptide from the supporting resin. The paper also describes an efficient route leading to asparagine-core pentasaccharide conjugate (3) which was prepared in seven steps for an overall yield of 23% from monosaccharide units 5, 6, 7 and 8. (C) 1997 Elsevier Science Ltd.

引用

页码：1917 / 1924

页数：8

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