Macrorestriction fragment profiles reveal genetic variation of Cowdria ruminantium isolates

被引：2

作者：

de Villiers, EP ^{[1
]}

Brayton, KA ^{[1
]}

Zweygarth, E ^{[1
]}

Allsopp, BA ^{[1
]}

机构：

[1] Onderstepoort Vet Inst, ZA-0110 Onderstepoort, South Africa

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 05期

关键词：

D O I：

10.1128/JCM.38.5.1967-1970.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal sizes ranging between 1,546 and 1,692 kb. Statistical analysis of the macrorestriction profiles indicated that all the isolates were distinct from each other; these data contribute to a better understanding of the epidemiology of this pathogen and may be exploited for the identification of genotype-specific DNA probes.

引用

页码：1967 / 1970

页数：4

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