Spectrophotometric analysis of flavonoid-DNA binding interactions at physiological conditions

被引：67

作者：

Janjua, Naveed Kausar ^{[1
]}

Siddiq, Asima ^{[2
]}

Yaqub, Azra ^{[1
]}

Sabahat, Sana ^{[1
]}

Qureshi, Rumana ^{[1
]}

ul Haque, Sayed ^{[3
]}

机构：

[1] Quaid I Azam Univ, Dept Chem, Islamabad 45230, Pakistan

[2] Quaid I Azam Univ, Natl Ctr Phys, Islamabad 45230, Pakistan

[3] Quaid I Azam Univ, Dept Biochem, Islamabad 45230, Pakistan

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2009年 / 74卷 / 05期

关键词：

Flavonoids; ds.DNA; Binding constants; Physiological conditions; Body temperature; SPECTROSCOPIC METHODS; QUERCETIN; DAMAGE; MORIN; BIOSENSORS; CLEAVAGE; RUTIN; DRUG;

D O I：

10.1016/j.saa.2009.09.022

中图分类号：

O433 [光谱学];

学科分类号：

070207 [光学];

摘要：

Mode of interactions of three flavonoids [morin (M), quercetin (Q), and rutin (R)] with chicken blood ds.DNA(ck.DNA)has been investigated spectrophotometrically at different temperatures including body temperature (310K) and at two physiological pH values, i.e. 7.4 (human blood pH) and 4.7 (stomach pH). The binding constants, K(f), evaluated using Benesi-Hildebrand equation showed that the flavonoids bind effectively through intercalation at both pH values and body temperature. Quercetin. somehow, showed greater binding capabilities with DNA. The free energies of flavonoid-DNA complexes indicated the spontaneity of their binding. The order of binding constants of three flavonoids at both pH values were found to be K(f(Q)) > K(f(R)) > K(f(M)) and at 310 K. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1135 / 1137

页数：3

共 16 条

[1]

Electrochemical and spectroscopic studies of the interaction of proflavine with DNA [J].