Strand Invasion of Extended, Mixed-Sequence B-DNA by γPNAs

被引：77

作者：

He, Gaofei

Rapireddy, Sirinivas

Bahal, Raman

Sahu, Bichismita

Ly, Danith H. ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 34期

基金：

美国国家卫生研究院;

关键词：

PEPTIDE NUCLEIC-ACID; DIETHYL PYROCARBONATE; DUPLEX DNA; RECOGNITION; BINDING; TRANSCRIPTION; DISPLACEMENT; POLYAMIDE; CYTOSINE; THYMINE;

D O I：

10.1021/ja900228j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this Communication, we show that peptide nucleic acids (PNAs) with lengths of 15-20 nucleotides, when preorganized into a right-handed helix, can invade mixed-sequence double-helical B-form DNA (B-DNA). Strand invasion occurs in a highly sequence-specific manner through direct Watson-Crick base pairing. Unlike the previously developed double-duplex invasion strategy, which requires simultaneous binding of two strands of pseudocomplementary PNAs to DNA, only a single strand of gamma PNA is required for invasion in this case, and no nucleobase substitution is needed.

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页码：12088 / +

页数：4

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