Chitosan-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible enzyme immobilization

被引：14

作者：

Arica, M. Yakup ^{[1
]}

Yilmaz, Meltem ^{[1
]}

Bayramoglu, Gulay ^{[1
]}

机构：

[1] Kirikkale Univ, Biochem Proc & Biomat Res Lab, Fac Sci, TR-71450 Kirikkale, Turkey

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2007年 / 103卷 / 05期

关键词：

membrane; contact angle; immobilized enzyme; adsorption; glucose oxidase;

D O I：

10.1002/app.25466

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Epoxy group-contain g poly(hydroxyethyl methacrylate/glycidyl methacrylate), p(HEMA/GMA), membrane was prepared by UV initiated photopolymerization. The membrane was grafted with chitosan (CH) and some of them were chelated with Fe(III) ions. The CH grafted, p(HEMA/GMA), and Fe(III) ions incorporated p(HEMA/ GMA)-CH-Fe(III) membranes were used for glucose oxidase (GOD) immobilization via adsorption. The maximum enzyme immobilization capacity of the p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes were 0.89 and 1.36 mg/mL, respectively. The optimal PH value for the immobilized GOD preparations is found to have shifted 0.5 units to more acidic PH 5.0. Optimum temperature for both immobilized preparations was 10 degrees C higher than that of the free enzyme and was significantly broader at higher temperatures. The apparent K-m values were found to be 6.9 and 5.8 mM for the adsorbed GOD on p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes, respectively. In addition, all the membranes surfaces were characterized by contact angle measurements. (c) 2006 Wiley Periodicals, Inc.

引用

页码：3084 / 3093

页数：10

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