XY body formation during rat spermatogenesis: an immunocytochemical study using antibodies against XY body-associated proteins

被引:12
作者
Kralewski, M [1 ]
Benavente, R [1 ]
机构
[1] UNIV WURZBURG,THEODOR BOVERI INST,BIOCTR,DEPT CELL & DEV BIOL,D-97074 WURZBURG,GERMANY
关键词
D O I
10.1007/s004120050251
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The process of formation of the XY body during meiotic prophase was investigated by immunocytochemistry on cryosections of pubertal rat testes using antibodies against three different XY body-associated proteins. Here we show that these proteins are detectable at only partially overlapping temporal windows. These findings provide the first evidence that the previously described morphological changes in the structure of the XY body that occur during meiotic prophase are accompanied by considerable changes in its protein composition.
引用
收藏
页码:304 / 307
页数:4
相关论文
共 14 条
[1]   Change of karyoskeleton during mammalian spermatogenesis: Expression pattern of nuclear lamin C2 and its regulation [J].
Alsheimer, M ;
Benavente, R .
EXPERIMENTAL CELL RESEARCH, 1996, 228 (02) :181-188
[2]   Molecular characterization and expression pattern of XY body-associated protein XY40 of the rat [J].
Alsheimer, M ;
Imamichi, Y ;
Heid, H ;
Benavente, R .
CHROMOSOMA, 1997, 106 (05) :308-314
[3]   Different strategies of X-inactivation in germinal and somatic cells: Histone H4 underacetylation does not mark the inactive X chromosome in the mouse male germline [J].
Armstrong, SJ ;
Hulten, MA ;
Keohane, AM ;
Turner, BM .
EXPERIMENTAL CELL RESEARCH, 1997, 230 (02) :399-402
[4]  
CLERMONT Y., 1959, ARCH ANAT MICROSC ET MORPHOL EXPTL, V48, P37
[5]   SEX DIFFERENCE IN METHYLATION OF SINGLE-COPY GENES IN HUMAN MEIOTIC GERM-CELLS - IMPLICATIONS FOR X-CHROMOSOME INACTIVATION, PARENTAL IMPRINTING, AND ORIGIN OF CPG MUTATIONS [J].
DRISCOLL, DJ ;
MIGEON, BR .
SOMATIC CELL AND MOLECULAR GENETICS, 1990, 16 (03) :267-282
[6]   THE MORPHOLOGICAL SEQUENCE OF XY-PAIRING IN THE NORWAY RAT RATTUS-NORVEGICUS [J].
JOSEPH, AM ;
CHANDLEY, AC .
CHROMOSOMA, 1984, 89 (05) :381-386
[7]   A novel Mr 77,000 protein of the XY body of mammalian spermatocytes: its localization in normal animals and in Searle's translocation carriers [J].
Kralewski, M ;
Novello, A ;
Benavente, R .
CHROMOSOMA, 1997, 106 (03) :160-167
[8]   THE GENE ENCODING A MAJOR COMPONENT OF THE LATERAL ELEMENTS OF SYNAPTONEMAL COMPLEXES OF THE RAT IS RELATED TO X-LINKED LYMPHOCYTE-REGULATED GENES [J].
LAMMERS, JHM ;
OFFENBERG, HH ;
VANAALDEREN, M ;
VINK, ACG ;
DIETRICH, AJJ ;
HEYTING, C .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (02) :1137-1146
[9]   Xist-deficient mice are defective in dosage compensation but not spermatogenesis [J].
Marahrens, Y ;
Panning, B ;
Dausman, J ;
Strauss, W ;
Jaenisch, R .
GENES & DEVELOPMENT, 1997, 11 (02) :156-166
[10]   SEX-CHROMOSOMES, RECOMBINATION, AND CHROMATIN CONFORMATION [J].
MCKEE, BD ;
HANDEL, MA .
CHROMOSOMA, 1993, 102 (02) :71-80