Effect of mitiglinide on Streptozotocin-induced experimental type 2 diabetic rats: A urinary metabonomics study based on ultra-performance liquid chromatography-tandem mass spectrometry

被引：19

作者：

Cai, Shuang ^{[1
,2
]}

Huo, Taoguang ^{[1
]}

Xu, Jinghua ^{[3
]}

Lu, Xiumei ^{[1
]}

Zheng, Shuning ^{[1
]}

Li, Famei ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Dept Analyt Chem, Shenyang 110016, Peoples R China

[2] China Med Univ, Affiliated Hosp 1, Shenyang 110001, Peoples R China

[3] Shenyang Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Shenyang 110016, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 29期

关键词：

Metabonomics; Type 2 diabetic rats; UPLC/MS; Mitiglinide; Biomarkers; MELLITUS PATIENTS; HPLC-TOF/MS; OBESE RATS; UPLC-MS; SPECTROSCOPY; METABOLOMICS; H-1-NMR; MOUSE; HYPERGLYCEMIA; METHODOLOGY;

D O I：

10.1016/j.jchromb.2009.08.044

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Mitiglinide is a new insulinotropic agent of the glinide class and its precise mechanism is not very clear yet. In this study, a urinary metabonomics method based on ultra-performance liquid chromatography-tandem mass spectrometry was developed to study the effect mechanism of mitiglinide on type 2 diabetes mellitus. With pattern recognition analysis of urinary metabolite profiles, a clear separation between Streptozotocin-induced type 2 diabetic rats and those treated with mitiglinide was achieved. Some significantly changed metabolites like citrate, creatinine, phenylalanine and bile acids (cholic acid, chenodeoxycholic acid and deoxycholic acid) have been identified and used to explain the mechanism. This work shows that metabonomics method is a valuable tool in drug mechanism study. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3619 / 3624

页数：6

共 40 条

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