The chloroplast protein BPG2 functions in brassinosteroid-mediated post-transcriptional accumulation of chloroplast rRNA

被引：57

作者：

Komatsu, Tomoyuki ^{[1
,2
]}

Kawaide, Hiroshi ^{[3
]}

Saito, Chieko ^{[4
]}

Yamagami, Ayumi ^{[1
]}

Shimada, Setsuko ^{[1
]}

Nakazawa, Miki ^{[5
]}

Matsui, Minami ^{[5
]}

Nakano, Akihiko ^{[4
,6
]}

Tsujimoto, Masafumi ^{[7
]}

Natsume, Masahiro ^{[3
]}

Abe, Hiroshi ^{[3
]}

Asami, Tadao ^{[7
,8
]}

Nakano, Takeshi ^{[1
,9
]}

机构：

[1] RIKEN, Adv Sci Inst, Plant Chem Biol Res Unit, Wako, Saitama 3510198, Japan

[2] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Fuchu, Tokyo 1838509, Japan

[3] Tokyo Univ Agr & Technol, Inst Symbiot Sci & Technol, Fuchu, Tokyo 1838509, Japan

[4] RIKEN, Adv Sci Inst, Mol Membrane Biol Lab, Wako, Saitama 3510198, Japan

[5] RIKEN, Plant Sci Ctr, Kanagawa 2300045, Japan

[6] Univ Tokyo, Dept Biol Sci, Grad Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[7] RIKEN, Cellular Biochem Lab, Adv Sci Inst, Wako, Saitama 3510198, Japan

[8] Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

[9] Japan Sci & Technol Agcy, Tokyo, Japan

来源：

PLANT JOURNAL | 2010年 / 61卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

brassinosteroid; BR biosynthesis inhibitor Brz; GTPase; chloroplast biogenesis; chloroplast rRNA; processing; GTP-BINDING PROTEIN; ARABIDOPSIS-THALIANA; BACILLUS-SUBTILIS; BIOSYNTHESIS INHIBITORS; CELL ELONGATION; MUTANT; GROWTH; GENE; CYTOCHROME-P450; LIGHT;

D O I：

10.1111/j.1365-313X.2009.04077.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Brassinazole (Brz) is a specific inhibitor of the biosynthesis of brassinosteroids (BRs), which regulate plant organ and chloroplast development. We identified a recessive pale green Arabidopsis mutant, bpg2-1 (Brz-insensitive-pale green 2-1) that showed reduced sensitivity to chlorophyll accumulation promoted by Brz in the light. BPG2 encodes a chloroplast-localized protein with a zinc finger motif and four GTP-binding domains that are necessary for normal chloroplast biogenesis. BPG2-homologous genes are evolutionally conserved in plants, green algae and bacteria. Expression of BPG2 is induced by light and Brz. Chloroplasts of the bpg2-1 mutant have a decreased number of stacked grana thylakoids. In bpg2-1 and bpg2-2 mutants, there was no reduction in expression of rbcL and psbA, but there was abnormal accumulation of precursors of chloroplast 16S and 23S rRNA. Chloroplast protein accumulation induced by Brz was suppressed by the bpg2 mutation. These results indicate that BPG2 plays an important role in post-transcriptional and translational regulation in the chloroplast, and is a component of BR signaling.

引用

页码：409 / 422

页数：14

共 42 条

[31] Isolation and characterization of a dominant negative mutant of Bacillus subtilis GTP-binding protein, YlqF, essential for biogenesis and maintenance of the 50 S ribosomal subunit [J].