Characterization of acquired resistance in lesion-mimic transgenic potato expressing bacterio-opsin

被引:41
作者
Abad, MS [1 ]
Hakimi, SM [1 ]
Kaniewski, WK [1 ]
Rommens, CMT [1 ]
Shulaev, V [1 ]
Lam, E [1 ]
Shah, DM [1 ]
机构
[1] RUTGERS STATE UNIV,COOK COLL,AGBIOTECH CTR,NEW BRUNSWICK,NJ 08903
关键词
D O I
10.1094/MPMI.1997.10.5.635
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The lesion-mimic mutants of certain plants display necrotic lesions resembling those of the hypersensitive response and activate local and systemic defense responses in the absence of pathogens, We have engineered a lesion-mimic phenotype in transgenic Russet Burbank potato plants through constitutive expression of a bacterio-opsin (bO) proton pump derived from Halobacterium halobium, Transgenic potato plants exhibiting a lesion-mimic phenotype had increased levels of salicylic acid and overexpressed several pathogenesis-related messenger RNAs, all hallmarks of systemic acquired resistance (SAR), The lesion-mimic plants also displayed enhanced resistance to the US1 isolate (A1 mating type) of a fungal pathogen, Phytophthora infestans, a causal agent of late blight disease, In contrast, little resistance was observed against the US8 isolate (A2 mating type) of this pathogen, Furthermore, a majority of the transgenic plants displaying the lesion-mimic phenotype had increased susceptibility to potato virus X. The tubers of these plants were not resistant to the bacterial pathogen Erwinia carotovora, These results indicate that expression of bO can result in the activation of defense responses in transgenic potato plants and show for the first time that bO expression can confer resistance to a pathogenic fungus, However, our results also demonstrate that like SAR, this ''engineered'' resistance is likely to be limited to certain pathogens and particular cultivars.
引用
收藏
页码:635 / 645
页数:11
相关论文
共 37 条
  • [31] Systemic acquired resistance
    Ryals, JA
    Neuenschwander, UH
    Willits, MG
    Molina, A
    Steiner, HY
    Hunt, MD
    [J]. PLANT CELL, 1996, 8 (10) : 1809 - 1819
  • [32] SAMBROOK J, 1908, MOL CLONING LAB MANU
  • [33] CHARACTERISTICS OF A STRONG PROMOTER FROM FIGWORT MOSAIC-VIRUS - COMPARISON WITH THE ANALOGOUS-35S PROMOTER FROM CAULIFLOWER MOSAIC-VIRUS AND THE REGULATED MANNOPINE SYNTHASE PROMOTER
    SANGER, M
    DAUBERT, S
    GOODMAN, RM
    [J]. PLANT MOLECULAR BIOLOGY, 1990, 14 (03) : 433 - 443
  • [34] DIFFERENTIAL ACCUMULATION OF MESSENGER-RNAS ENCODING EXTRACELLULAR AND INTRACELLULAR PR PROTEINS IN TOMATO INDUCED BY VIRULENT AND AVIRULENT RACES OF CLADOSPORIUM-FULVUM
    VANKAN, JAL
    JOOSTEN, MHAJ
    WAGEMAKERS, CAM
    VANDENBERGVELTHUIS, GCM
    DEWIT, PJGM
    [J]. PLANT MOLECULAR BIOLOGY, 1992, 20 (03) : 513 - 527
  • [35] DISEASE RESISTANCE CONFERRED BY EXPRESSION OF A GENE ENCODING H2O2-GENERATING GLUCOSE-OXIDASE IN TRANSGENIC POTATO PLANTS
    WU, GS
    SHORTT, BJ
    LAWRENCE, EB
    LEVINE, EB
    FITZSIMMONS, KC
    SHAH, DM
    [J]. PLANT CELL, 1995, 7 (09) : 1357 - 1368
  • [36] ENDOGENOUS SALICYLIC-ACID LEVELS CORRELATE WITH ACCUMULATION OF PATHOGENESIS-RELATED PROTEINS AND VIRUS-RESISTANCE IN TOBACCO
    YALPANI, N
    SHULAEV, V
    RASKIN, I
    [J]. PHYTOPATHOLOGY, 1993, 83 (07) : 702 - 708
  • [37] ACTIVATION OF 2 OSMOTIN-LIKE PROTEIN GENES BY ABIOTIC STIMULI AND FUNGAL PATHOGEN IN TRANSGENIC POTATO PLANTS
    ZHU, BL
    CHEN, THH
    LI, PH
    [J]. PLANT PHYSIOLOGY, 1995, 108 (03) : 929 - 937