RNA polymerase II-TFIIB structure and mechanism of transcription initiation

被引：239

作者：

Kostrewa, Dirk ^{[1
,2
]}

Zeller, Mirijam E. ^{[3
]}

Armache, Karim-Jean ^{[1
,2
]}

Seizl, Martin ^{[1
,2
]}

Leike, Kristin ^{[1
,2
]}

Thomm, Michael ^{[3
]}

Cramer, Patrick ^{[1
,2
]}

机构：

[1] Univ Munich, Gene Ctr Munich, D-81377 Munich, Germany

[2] Univ Munich, Dept Chem & Biochem, CIPSM, D-81377 Munich, Germany

[3] Univ Regensburg, Inst Biochem Genet & Mikrobiol, D-93053 Regensburg, Germany

来源：

NATURE | 2009年 / 462卷 / 7271期

关键词：

START SITE SELECTION; N-TERMINAL REGION; PREINITIATION COMPLEX; ANGSTROM RESOLUTION; ELONGATION COMPLEX; CRYSTAL-STRUCTURE; SACCHAROMYCES-CEREVISIAE; ZINC RIBBON; YEAST; DNA;

D O I：

10.1038/nature08548

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To initiate gene transcription, RNA polymerase II (Pol II) requires the transcription factor IIB (B). Here we present the crystal structure of the complete Pol II-B complex at 4.3 angstrom resolution, and complementary functional data. The results indicate the mechanism of transcription initiation, including the transition to RNA elongation. Promoter DNA is positioned over the Pol II active centre cleft with the 'B-core' domain that binds the wall at the end of the cleft. DNA is then opened with the help of the 'B-linker' that binds the Pol II rudder and clamp coiled-coil at the edge of the cleft. The DNA template strand slips into the cleft and is scanned for the transcription start site with the help of the 'B-reader' that approaches the active site. Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation.

引用

页码：323 / 330

页数：8

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