Quantitative interrogation of micropatterned biomolecules by surface force microscopy

被引：15

作者：

Garrison, MD ^{[1
]}

McDevitt, TC ^{[1
]}

Luginbühl, R ^{[1
]}

Giachelli, CM ^{[1
]}

Stayton, P ^{[1
]}

Ratner, BD ^{[1
]}

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

来源：

ULTRAMICROSCOPY | 2000年 / 82卷 / 1-4期

关键词：

scanning force microscopy; microcontact printing; osteopontin; biomaterials;

D O I：

10.1016/S0304-3991(99)00150-3

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Synthetic biomaterials are widely used in medical implants with success in improving and extending quality of life. However, these materials were not originally designed to interact with cells through specific signaling pathways. As a result, the interaction with the body is mediated through passive adsorption of a disorganized protein monolayer. Next generation biomaterials have been proposed to be active in modifying the biological response of the host through the incorporation of specific biorecognition moieties. An important tool in the development of these novel active biomaterials is the scanning force microscope (SFM). The SFM allows for interrogation of bioactive biomaterials in mapping or spectroscopic modes. In this work, micropatterned protein surfaces were prepared using biomolecules implicated in wound healing. The surfaces were imaged via SFM and the specific binding forces between surface associated biomolecules and antibody functionalized tips were quantified. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

引用

页码：193 / 202

页数：10

共 36 条

[1] Detection of antigen-antibody binding events with the atomic force microscope [J].