Comparison of multiplexed techniques for detection of bacterial and viral proteins

被引：51

作者：

Rao, RS

Visuri, SR

McBride, MT

Albala, JS

Matthews, DL

Coleman, MA

机构：

[1] Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA

[2] Univ Calif Davis, Dept Biomed Engn, Davis, CA 95616 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2004年 / 3卷 / 04期

关键词：

microarray; multiplex assay; bead-based assay; Luminex; detection; immunoassay;

D O I：

10.1021/pr034130t

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.

引用

页码：736 / 742

页数：7

共 20 条

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