Structure of the Ras-binding domain of RalGEF and implications for Ras binding and signalling

被引：107

作者：

Geyer, M

Herrmann, C

Wohlgemuth, S

Wittinghofer, A

Kalbitzer, HR

机构：

[1] MAX PLANCK INST MED RES, BIOPHYS ABT, D-69120 HEIDELBERG, GERMANY

[2] MAX PLANCK INST MOL PHYSIOL, ABT STRUKTURELLE BIOL, D-44139 DORTMUND, GERMANY

来源：

NATURE STRUCTURAL BIOLOGY | 1997年 / 4卷 / 09期

关键词：

D O I：

10.1038/nsb0997-694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The solution structure of the Ras-binding domain (RED) of pal guanine-nucleotide exchange factor RalGEF was solved by NMR spectroscopy. The overall structure is similar to that of Raf-RBD, another effector of pas, although the sequence identity is only 13%. N-15 chemical shifts changes in the complex of RalGEF-RBD with Ras indicate an interaction similar to the intermolecular beta-sheet observed for the complex between Ras and Raf-RBD.

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页码：694 / 699

页数：6

相关论文

共 30 条

[1] CHARACTERIZATION OF A GUANINE-NUCLEOTIDE DISSOCIATION STIMULATOR FOR A RAS-RELATED GTPASE [J].

ALBRIGHT, CF ;

GIDDINGS, BW ;

LIU, J ;

VITO, M ;

WEINBERG, RA .

EMBO JOURNAL, 1993, 12 (01) :339-347

[2]

BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0

[3]

BRUNGER AT, 1992, XPLOR VERSION 3 1

[4] ASSIGNMENT OF COMPLEX H-1-NMR SPECTRA VIA TWO-DIMENSIONAL HOMONUCLEAR HARTMANN-HAHN SPECTROSCOPY [J].

DAVIS, DG ;

BAX, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (09) :2820-2821

[5] SOLUTION STRUCTURE OF THE RAS-BINDING DOMAIN OF C-RAF-1 AND IDENTIFICATION OF ITS RAS INTERACTION SURFACE [J].

EMERSON, SD ;

MADISON, VS ;

PALERMO, RE ;

WAUGH, DS ;

SCHEFFLER, JE ;

TSAO, KL ;

KIEFER, SE ;

LIU, SP ;

FRY, DC .

BIOCHEMISTRY, 1995, 34 (21) :6911-6918

[6] Evidence for a Ras/Ral signaling cascade [J].

Feig, LA ;

Urano, T ;

Cantor, S .

TRENDS IN BIOCHEMICAL SCIENCES, 1996, 21 (11) :438-441

[7] Conformational transitions in p21(ras) and in its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP [J].

Geyer, M ;

Schweins, T ;

Herrmann, C ;

Prisner, T ;

Wittinghofer, A ;

Kalbitzer, HR .

BIOCHEMISTRY, 1996, 35 (32) :10308-10320

[8] Relax, a flexible program for the back calculation of NOESY spectra based on complete-relaxation-matrix formalism [J].

Gorler, A ;

Kalbitzer, HR .

JOURNAL OF MAGNETIC RESONANCE, 1997, 124 (01) :177-188

[9] Differential interaction of the Ras family GTP-binding proteins H-Ras, Rap1A, and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor [J].

Herrmann, C ;

Horn, G ;

Spaargaren, M ;

Wittinghofer, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (12) :6794-6800

[10] INVESTIGATION OF EXCHANGE PROCESSES BY 2-DIMENSIONAL NMR-SPECTROSCOPY [J].

JEENER, J ;

MEIER, BH ;

BACHMANN, P ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1979, 71 (11) :4546-4553