Deficiency in fatty acid synthase leads to premature cell death and dramatic alterations in plant morphology

被引:198
作者
Mou, ZL [1 ]
He, YK [1 ]
Dai, Y [1 ]
Liu, XF [1 ]
Li, JY [1 ]
机构
[1] Acad Sinica, Inst Genet, Beijing 100101, Peoples R China
关键词
D O I
10.1105/tpc.12.3.405
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An Arabidopsis mosaic deafh1 (mod1) mutant, which has premature cell death in multiple organs, was isolated, mod1 plants display multiple morphological phenotypes, including chlorotic and curly leaves, distorted siliques, premature senescence of primary inflorescences, reduced fertility, and semidwarfism. The phenotype of the mod1 mutant results from a single nuclear recessive mutation, and the MODI gene was isolated by using a map-based cloning approach. The MOD1 gene encodes an enoyl-acyl carrier protein (ACP) reductase, which is a subunit of the fatty acid synthase complex that catalyzes de novo synthesis of fatty acids. An amino acid substitution in the enoyl-ACP reductase of the mod1 mutant causes a marked decrease in its enzymatic activity, impairing fatty acid biosynthesis and decreasing the amount of total lipids in mod1 plants, These results demonstrate that a deficiency in fatty acid biosynthesis has pleiotropic effects on plant growth and development and causes premature cell death.
引用
收藏
页码:405 / 417
页数:13
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共 64 条
[21]  
Gibson S. I., 1992, Methods in Arabidopsis research., P119
[22]   A novel suppressor of cell death in plants encoded by the Lls1 gene of maize [J].
Gray, J ;
Close, PS ;
Briggs, SP ;
Johal, GS .
CELL, 1997, 89 (01) :25-31
[23]   Programmed cell death: A way of life for plants [J].
Greenberg, JT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (22) :12094-12097
[24]   PROGRAMMED CELL-DEATH IN PLANTS - A PATHOGEN-TRIGGERED RESPONSE ACTIVATED COORDINATELY WITH MULTIPLE DEFENSE FUNCTIONS [J].
GREENBERG, JT ;
GUO, AL ;
KLESSIG, DF ;
AUSUBEL, FM .
CELL, 1994, 77 (04) :551-563
[25]   SULFONYLUREA-RESISTANT MUTANTS OF ARABIDOPSIS-THALIANA [J].
HAUGHN, GW ;
SOMERVILLE, C .
MOLECULAR & GENERAL GENETICS, 1986, 204 (03) :430-434
[26]   A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize [J].
Hu, GS ;
Yalpani, N ;
Briggs, SP ;
Johal, GS .
PLANT CELL, 1998, 10 (07) :1095-1105
[27]   A recessive heterochronic mutation, plastochron1, shortens the plastochron and elongates the vegetative phase in rice [J].
Itoh, JI ;
Hasegawa, A ;
Kitano, H ;
Nagato, Y .
PLANT CELL, 1998, 10 (09) :1511-1521
[28]   INDUCTION OF APOPTOTIC DNA-DAMAGE AND CELL-DEATH BY ACTIVATION OF THE SPHINGOMYELIN PATHWAY [J].
JARVIS, WD ;
KOLESNICK, RN ;
FORNARI, FA ;
TRAYLOR, RS ;
GEWIRTZ, DA ;
GRANT, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :73-77
[29]   Resistance of Nicotiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF1 [J].
Kamoun, S ;
van West, P ;
Vleeshouwers, VGAA ;
de Groot, KE ;
Govers, F .
PLANT CELL, 1998, 10 (09) :1413-1425
[30]   THE USE OF A HYBRID GENETIC SYSTEM TO STUDY THE FUNCTIONAL-RELATIONSHIP BETWEEN PROKARYOTIC AND PLANT MULTIENZYME FATTY-ACID SYNTHETASE COMPLEXES [J].
KATER, MM ;
KONINGSTEIN, GM ;
NIJKAMP, HJJ ;
STUITJE, AR .
PLANT MOLECULAR BIOLOGY, 1994, 25 (05) :771-790