Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat

被引:61
作者
Crismani, Wayne
Baumann, Ute
Sutton, Tim
Shirley, Neil
Webster, Tracie
Spangenberg, German
Langridge, Peter
Able, Jason A.
机构
[1] Univ Adelaide, Sch Agr Food & Wine, Mol Plant Breedign Cooperat Res Ctr, Glen Osmond, SA 5064, Australia
[2] Univ Adelaide, Sch Agr Food & Wine, Australian Ctr Plant Funct Genom, Glen Osmond, SA 5064, Australia
[3] La Trobe Univ, Mol Plant Breeding Coopeat Res Ctr, Bundoora, Vic 3083, Australia
[4] La Trobe Univ, Australian Ctr Plant Funct Genom, Bundoora, Vic 3083, Australia
关键词
D O I
10.1186/1471-2164-7-267
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Our understanding of the mechanisms that govern the cellular process of meiosis is limited in higher plants with polyploid genomes. Bread wheat is an allohexaploid that behaves as a diploid during meiosis. Chromosome pairing is restricted to homologous chromosomes despite the presence of homoeologues in the nucleus. The importance of wheat as a crop and the extensive use of wild wheat relatives in breeding programs has prompted many years of cytogenetic and genetic research to develop an understanding of the control of chromosome pairing and recombination. The rapid advance of biochemical and molecular information on meiosis in model organisms such as yeast provides new opportunities to investigate the molecular basis of chromosome pairing control in wheat. However, building the link between the model and wheat requires points of data contact. Results: We report here a large-scale transcriptomics study using the Affymetrix wheat GeneChip((R)) aimed at providing this link between wheat and model systems and at identifying early meiotic genes. Analysis of the microarray data identified 1,350 transcripts temporally-regulated during the early stages of meiosis. Expression profiles with annotated transcript functions including chromatin condensation, synaptonemal complex formation, recombination and fertility were identified. From the 1,350 transcripts, 30 displayed at least an eight-fold expression change between and including pre-meiosis and telophase II, with more than 50% of these having no similarities to known sequences in NCBI and TIGR databases. Conclusion: This resource is now available to support research into the molecular basis of pairing and recombination control in the complex polyploid, wheat.
引用
收藏
页数:17
相关论文
共 54 条
[1]   Arabidopsis RAD51C gene is important for homologous recombination in meiosis and mitosis [J].
Abe, K ;
Osakabe, K ;
Nakayama, S ;
Endo, M ;
Tagiri, A ;
Todoriki, S ;
Ichikawa, H ;
Toki, S .
PLANT PHYSIOLOGY, 2005, 139 (02) :896-908
[2]   Genes involved in organ separation in Arabidopsis: An analysis of the cup-shaped cotyledon mutant [J].
Aida, M ;
Ishida, T ;
Fukaki, H ;
Fujisawa, H ;
Tasaka, M .
PLANT CELL, 1997, 9 (06) :841-857
[3]   Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis [J].
Andrews, J ;
Bouffard, GG ;
Cheadle, C ;
Lü, JN ;
Becker, KG ;
Oliver, B .
GENOME RESEARCH, 2000, 10 (12) :2030-2043
[4]   The role of Aquaporins and membrane damage in chilling and hydrogen peroxide induced changes in the hydraulic conductance of maize roots [J].
Aroca, R ;
Amodeo, G ;
Fernández-Illescas, S ;
Herman, EM ;
Chaumont, F ;
Chrispeels, MJ .
PLANT PHYSIOLOGY, 2005, 137 (01) :341-353
[5]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[6]  
BENNETT M D, 1971, Proceedings of the Royal Society of London Series B Biological Sciences, V178, P277
[7]   TIME AND DURATION OF FEMALE MEIOSIS IN WHEAT, RYE AND BARLEY [J].
BENNETT, MD ;
FINCH, RA ;
SMITH, JB ;
RAO, MK .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1973, 183 (1072) :301-+
[8]  
BENNETT MD, 1971, PROC R SOC SER B-BIO, V178, P259, DOI 10.1098/rspb.1971.0065
[9]   EFFECTS OF POLYPLOIDY ON MEIOTIC DURATION AND POLLEN DEVELOPMENT IN CEREAL ANTHERS [J].
BENNETT, MD ;
SMITH, JB .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1972, 181 (1062) :81-+
[10]   Differing requirements for the Arabidopsis Rad51 paralogs in meiosis and DNA repair [J].
Bleuyard, JY ;
Gallego, ME ;
Savigny, F ;
White, CI .
PLANT JOURNAL, 2005, 41 (04) :533-545