Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples

被引：52

作者：

Long, F. ^{[1
]}

Shi, H. C. ^{[1
]}

He, M. ^{[1
]}

Sheng, J. W. ^{[1
]}

Wang, J. F. ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Environm Sci & Engn, Environm Simulate & Pollut Control State Key Join, Beijing 100084, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2009年 / 649卷 / 01期

关键词：

Chemiluminescence; Enzyme immunoassay; Microcystin-LR; Environmental analysis; ANTIBODIES; EXPOSURE;

D O I：

10.1016/j.aca.2009.07.026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive, specific, simple, and rapid chemiluminescence enzyme immunoassay (CLEIA) was developed for the determination of microcystin-LR (MC-LR). Several physicochemical parameters such as the chemiluminescent assay mediums, the dilution ratio of MC-LR-OVA conjugate, monoclonal antibody concentration, and peroxidase labeled antibody concentration were studied and optimized. Under optimum conditions, calibration curve obtained for MC-LR had detection limits of 0.032 +/- 0.003 mu g L-1. the 50% inhibition concentration (IC50) was 0.20 +/- 0.02 mu g L-1 and the quantitative detection range was 0.062-0.65 mu g L-1. The proposed methods was successfully applied to the monitoring of MC-LR in spiked water samples without significant effect of the matrix, and the recovery of MC-LR added to water samples at different concentrations ranged from 80% to 115% with the coefficients of variation (CVs) less than 9%. The LOD attained from the calibration curves and the results obtained for the real samples demonstrate the potential use of CLEIA as a screening tool for the analysis of MC-LR in environmental samples. (C) 2009 Published by Elsevier B.V.

引用

页码：123 / 127

页数：5

共 25 条

[1] High-throughput automated luminescent magnetic particle-based immunoassay to monitor human exposure to pyrethroid insecticides [J].