Synthesis by an alpha-glucosidase of glycosyl-trehaloses with an isomaltosyl residue

被引：27

作者：

Kurimoto, M ^{[1
]}

Nishimoto, T ^{[1
]}

Nakada, T ^{[1
]}

Chaen, H ^{[1
]}

Fukuda, S ^{[1
]}

Tsujisaka, Y ^{[1
]}

机构：

[1] HAYASHIBARA INST CORP, OKAYAMA 700, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 04期

关键词：

trehalose; isomaltosyl residue; alpha-glucosidase;

D O I：

10.1271/bbb.61.699

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosyl-trehaloses with an isomaltosyl residue were synthesized by alpha-glucosidase from Aspergillus niger by using maltotetraose as a glucosyl donor and trehalose as the acceptor, The one trisaccharide and two tetrasaccharides formed mere isolated by successive column chromatography, The results of an enzymatic digestion, methylation analysis, and C-13-NMR studies indicated that these oligosaccharides were alpha-isomaltosyl alpha-glucoside, alpha-isomaltotriosyl alpha-glucoside and alpha-isomaltosyl alpha-isomaltoside. These oligosaccharides were not fermented to an acid by Streptococcus mutans, and they effectively inhibited water-insoluble glucan synthesis from sucrose by glucosyltransferase, In an in vitro utilization test with human intestinal bacteria, these oligosaccharides were predominantly utilized by Bifidobacteria.

引用

页码：699 / 703

页数：5

共 24 条

[1] REGIOSELECTIVE SYNTHESES OF TREHALOSE-CONTAINING TRISACCHARIDES USING VARIOUS GLYCOHYDROLASES [J].