ENZYMATIC PREPARATION OF NOVEL NONREDUCING OLIGOSACCHARIDES HAVING AN ISOMALTOSYL RESIDUE BY USING THE TRANSFER ACTION OF ISOMALTODEXTRANASE FROM ARTHROBACTER-GLOBIFORMIS T6

被引：9

作者：

KIM, YK

TSUMURAYA, Y

SAKANO, Y

机构：

[1] TOKYO UNIV AGR & TECHNOL, FAC AGR, DEPT APPL BIOL SCI, FUCHU, TOKYO 183, JAPAN

[2] SAITAMA UNIV, FAC SCI, DEPT BIOCHEM, URAWA, SAITAMA 338, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 07期

关键词：

D O I：

10.1271/bbb.59.1367

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-reducing oligosaccharides having an isomaltosyl residue, various alkyl alpha-isomaltosides, 6(G)-alpha-isomaltosyl sucrose, and 6-alpha-isomaltosyl trehalose, were prepared in more than 30% yields using the transfer action of isomaltodextranase (EC 3.2.1.94) from Arthrobacter globiformis T6. 6(G)-alpha-Isomaltosyl sucrose and 6-alpha-isomaltosyl trehalose were hydrolyzed by isomaltodextranase more easily than alkyl alpha-isomaltosides. The structure of these transfer products were identified by the hydrolysis of isomaltodextranase, reducing end analysis, methylation analysis, and H-1-NMR analysis.

引用

页码：1367 / 1369

页数：3

共 24 条

[1] STUDY OF C-13H COUPLING-CONSTANTS IN HEXOPYRANOSES [J].