Electric potential control of DNA immobilization on gold electrode

被引：52

作者：

Ge, CW ^{[1
]}

Liao, JH ^{[1
]}

Yu, W ^{[1
]}

Gu, N ^{[1
]}

机构：

[1] Southeast Univ, Natl Lab Mol & Biomol Elect, Nanjing 210096, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DNA immobilize; controlled potential; DNA-based device;

D O I：

10.1016/S0956-5663(02)00115-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The assembly of synthetic, controllable molecules is one of the goals in nanotechnology. The primary objective of this contribution is to selectively immobilize DNA on gold via electric potential control. The self-assembly monolayer (SAM) was prepared with 2-aminoethanethiol (AET) on the gold electrode. A new approach based on electric potential was firstly used to control DNA immobilization covalently onto the SAM with the activation of 1-ethyl-3(3-dimethyl-aminopropyl)-carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS) in low ionic strength solution. The influence of electric potential on DNA immobilization was investigated by means of cyclic voltammogram, A.C. impedance, auger electron spectrometer as well as atomic force microscope (AFM) on template-stripped gold surface. The result proves that controlled potential can affect the course of DNA immobilization. More negative potential can restrain the DNA immobilization, while the more positive potential can accelerate the DNA immobilization. It is of great significance for the control of DNA self-assembly and will find wide application in the fields of DNA-based devices. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：53 / 58

页数：6

共 21 条

[1]

BARD AJ, 1980, FUNDAMENTAL APPL, pCH9

[2] DNA transistor and quantum bit element: Realization of nano-biomolecular logical devices [J].

Ben-Jacob, E ;

Hermon, Z ;

Caspi, S .

PHYSICS LETTERS A, 1999, 263 (03) :199-202

[3]

Boukamp B. A., 1993, EQUIVALENT CIRCUITS

[4] DNA-templated assembly and electrode attachment of a conducting silver wire [J].

Braun, E ;

Eichen, Y ;

Sivan, U ;

Ben-Yoseph, G .

NATURE, 1998, 391 (6669) :775-778

[5] VOLTAMMETRIC STUDIES OF THE INTERACTION OF METAL-CHELATES WITH DNA .2. TRIS-CHELATED COMPLEXES OF COBALT(III) AND IRON(II) WITH 1,10-PHENANTHROLINE AND 2,2'-BIPYRIDINE [J].

CARTER, MT ;

RODRIGUEZ, M ;

BARD, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (24) :8901-8911

[6] STUDIES ON INTERACTION OF ANTHRACYCLINE ANTIBIOTICS AND DEOXYRIBONUCLEIC-ACID - EQUILIBRIUM BINDING-STUDIES ON INTERACTION OF DAUNOMYCIN WITH DEOXYRIBONUCLEIC-ACID [J].

CHAIRES, JB ;

DATTAGUPTA, N ;

CROTHERS, DM .

BIOCHEMISTRY, 1982, 21 (17) :3933-3940

[7] POLYMER-BOUND EDC (P-EDC) - A CONVENIENT REAGENT FOR FORMATION OF AN AMIDE BOND [J].

DESAI, MC ;

STRAMIELLO, LMS .

TETRAHEDRON LETTERS, 1993, 34 (48) :7685-7688

[8] OPTICALLY-ACTIVE COORDINATION-COMPOUNDS .32. POTASSIUM(+)TRIS-[L-CYSTEINESULPHINATO(2-)-SN]COBALTATE(III) - VERSATILE AGENT FOR RESOLUTION OF 3+ SPECIES [J].

DOLLIMORE, LS ;

GILLARD, RD .

JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS, 1973, (09) :933-940

[9] Electrical conduction through DNA molecules [J].

Fink, HW ;

Schönenberger, C .

NATURE, 1999, 398 (6726) :407-410

[10] ATOMIC FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA [J].

HANSMA, HG ;

SINSHEIMER, RL ;

LI, MQ ;

HANSMA, PK .

NUCLEIC ACIDS RESEARCH, 1992, 20 (14) :3585-3590

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