TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila

被引：172

作者：

Hochheimer, A ^{[1
]}

Zhou, S ^{[1
]}

Zheng, S ^{[1
]}

Holmes, MC ^{[1
]}

Tjian, R ^{[1
]}

机构：

[1] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

NATURE | 2002年 / 420卷 / 6914期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nature01167

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Drosophila TATA-box-binding protein (TBP)-related factor 2 (TRF2) is a member of a family of TBP-related factors present in metazoan organisms. Recent evidence suggests that TRF2s are required for proper embryonic development and differentiation(1-5). However, true target promoters and the mechanisms by which TRF2 operates to control transcription remain elusive. Here we report the antibody affinity purification of a Drosophila TRF2-containing complex that contains components of the nucleosome remodelling factor (NURF) chromatin remodelling complex as well as the DNA replication-related element (DRE)-binding factor DREF. This latter finding led us to potential target genes containing TRF2-responsive promoters. We have used a combination of in vitro and in vivo assays to show that the DREF-containing TRF2 complex directs core promoter recognition of the proliferating cell nuclear antigen (PCNA) gene. We also identified additional TRF2-responsive target genes involved in DNA replication and cell proliferation. These data suggest that TRF2 functions as a core promoter-selectivity factor responsible for coordinating transcription of a subset of genes in Drosophila.

引用

页码：439 / 445

页数：8

共 29 条

[21] TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family [J].

Rabenstein, MD ;

Zhou, S ;

Lis, JT ;

Tjian, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (09) :4791-4796

[22] Core promoters: active contributors to combinatorial gene regulation [J].

Smale, ST .

GENES & DEVELOPMENT, 2001, 15 (19) :2503-2508

[23] A TRF1:BRF complex directs Drosophila RNA polymerase III transcription [J].

Takada, S ;

Lis, JT ;

Zhou, S ;

Tjian, R .

CELL, 2000, 101 (05) :459-469

[24] ISWI, A MEMBER OF THE SW12/SNF2 ATPASE FAMILY, ENCODES THE 140-KDA SUBUNIT OF THE NUCLEOSOME REMODELING FACTOR [J].

TSUKIYAMA, T ;

DANIEL, C ;

TAMKUN, J ;

WU, C .

CELL, 1995, 83 (06) :1021-1026

[25] Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II [J].

VargaWeisz, PD ;

Wilm, M ;

Bonte, E ;

Dumas, K ;

Mann, M ;

Becker, PB .

NATURE, 1997, 388 (6642) :598-602

[26] Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus [J].

Veenstra, GJC ;

Weeks, DL ;

Wolffe, AP .

SCIENCE, 2000, 290 (5500) :2312-+

[27] Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions [J].

Xiao, H ;

Sandaltzopoulos, R ;

Wang, HM ;

Hamiche, A ;

Ranallo, R ;

Lee, KM ;

Fu, DG ;

Wu, C .

MOLECULAR CELL, 2001, 8 (03) :531-543

[28] Roles of multiple promoter elements of the proliferating cell nuclear antigen gene during Drosophila development [J].

Yamaguchi, M ;

Hirose, F ;

Matsukage, A .

GENES TO CELLS, 1996, 1 (01) :47-58

[29] Spermiogenesis deficiency in mice lacking the Trf2 gene [J].

Zhang, D ;

Penttila, TL ;

Morris, PL ;

Teichmann, M ;

Roeder, RG .

SCIENCE, 2001, 292 (5519) :1153-1155

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