Solid-phase enzyme activity assay utilizing an entrapped fluorescence-signaling DNA aptamer

被引：55

作者：

Rupcich, Nicholas

Nutiu, Razvan

Li, Yingfu

Brennan, John D.

机构：

[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada

[2] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 20期

关键词：

aptamer; enzymes; fluorescence; inhibitors; sol-gel processes;

D O I：

10.1002/anie.200504576

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) Layered enzyme assays: The use of a layered sol-gel based material allows for spatial separation of coimmobilized enzyme and fluorescent aptamer reporter species. This provides a route for facile assaying of enzyme activity and inhibition in cases where the aptamer signals the presence of the enzyme substrate. This then results in accurate IC50 determinations. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：3295 / 3299

页数：5

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