Streptococcus aureus infection of mouse or human osteoblasts induces high levels of interleukin-6 and interleukin-12 production

被引:105
作者
Bost, KL
Ramp, WK
Nicholson, NC
Bento, JL
Marriott, I
Hudson, MC
机构
[1] Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA
[2] Carolinas Med Ctr, Orthopaed Res Lab, Charlotte, NC 28203 USA
关键词
D O I
10.1086/315138
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Staphylococcus aureus is the principal causative agent of the inflammatory bone disease osteomyelitis. Unfortunately, the pathogenesis of this often chronic infection is poorly understood and is complicated by the recent observation that bone-forming osteoblasts can harbor S. aureus. Such an infection presents a significant challenge for the host immune response, because osteoblasts are not known to initiate protective cell-mediated immune responses. Cultured mouse and human osteoblasts infected with S. aureus were found to express high levels of interleukin (IL)-6 and IL-12p75, on the basis of complementary investigations demonstrating both S. aureus-induced up-regulation of expression of IL-6 and IL-12p40 mRNA and secretion of IL-6 and IL-12p75 by these cells. Additionally, a quantitative bioassay demonstrated that IL-12p75 secreted after infection was biologically active. These studies are the first to demonstrate induced IL-12p75 expression by osteoblasts and suggest a previously unrecognized role for osteoblasts in initiating immune responses after S. aureus infection.
引用
收藏
页码:1912 / 1920
页数:9
相关论文
共 68 条
[21]   PRODUCTION OF TUMOR NECROSIS FACTOR BY HUMAN OSTEOBLASTS IS MODULATED BY OTHER CYTOKINES, BUT NOT BY OSTEOTROPIC HORMONES [J].
GOWEN, M ;
CHAPMAN, K ;
LITTLEWOOD, A ;
HUGHES, D ;
EVANS, D ;
RUSSELL, G .
ENDOCRINOLOGY, 1990, 126 (02) :1250-1255
[22]   ADENYL-CYCLASE AND INTERLEUKIN-6 ARE DOWNSTREAM EFFECTORS OF PARATHYROID-HORMONE RESULTING IN STIMULATION OF BONE-RESORPTION [J].
GREENFIELD, EM ;
SHAW, SM ;
GORNIK, SA ;
BANKS, MA .
JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (03) :1238-1244
[23]   THE ISOLATION AND CULTURE OF CELLS FROM EXPLANTS OF HUMAN TRABECULAR BONE [J].
GUNDLE, R ;
BERESFORD, JN .
CALCIFIED TISSUE INTERNATIONAL, 1995, 56 :S8-S10
[24]   RECOMBINANT INTERLEUKIN-12 CURES MICE INFECTED WITH LEISHMANIA-MAJOR [J].
HEINZEL, FP ;
SCHOENHAUT, DS ;
RERKO, RM ;
ROSSER, LE ;
GATELY, MK .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (05) :1505-1509
[25]  
Heinzel FP, 1997, J IMMUNOL, V158, P4381
[26]   FIBRONECTIN, FIBRINOGEN, AND LAMININ ACT AS MEDIATORS OF ADHERENCE OF CLINICAL STAPHYLOCOCCAL ISOLATES TO FOREIGN MATERIAL [J].
HERRMANN, M ;
VAUDAUX, PE ;
PITTET, D ;
AUCKENTHALER, R ;
LEW, PD ;
SCHUMACHERPERDREAU, F ;
PETERS, G ;
WALDVOGEL, FA .
JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (04) :693-701
[27]   Intercellular chatter: Osteoblasts, osteoclasts and interleukin 6 [J].
Hofbauer, LC ;
Heufelder, AE .
EUROPEAN JOURNAL OF ENDOCRINOLOGY, 1996, 134 (04) :425-426
[28]   DEVELOPMENT OF TH1 CD4+ T-CELLS THROUGH IL-12 PRODUCED BY LISTERIA-INDUCED MACROPHAGES [J].
HSIEH, CS ;
MACATONIA, SE ;
TRIPP, CS ;
WOLF, SF ;
OGARRA, A ;
MURPHY, KM .
SCIENCE, 1993, 260 (5107) :547-549
[29]   Internalization of Staphylococcus aureus by cultured osteoblasts [J].
Hudson, MC ;
Ramp, WK ;
Nicholson, NC ;
Williams, AS ;
Nousiainen, MT .
MICROBIAL PATHOGENESIS, 1995, 19 (06) :409-419
[30]  
ISHIMI Y, 1990, J IMMUNOL, V145, P3297