Metabolomic profiling of rat serum associated with isoproterenol-induced myocardial infarction using ultra-performance liquid chromatography/time-of-flight mass spectrometry and multivariate analysis

被引：64

作者：

Zhang, Hong-yang ^{[1
,2
]}

Chen, Xi ^{[2
]}

Hu, Ping ^{[1
]}

Liang, Qiong-lin ^{[2
]}

Liang, Xiao-ping ^{[1
,2
]}

Wang, Yi-ming ^{[2
]}

Luo, Guo-an ^{[1
,2
]}

机构：

[1] E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

[2] Tsinghua Univ, Key Lab Biorgan Phosphorus Chem & Chem Biol, Dept Chem, Beijing 100084, Peoples R China

来源：

TALANTA | 2009年 / 79卷 / 02期

关键词：

Metabolomics; Ultra-performance liquid chromatography/time-of-flight mass; spectrometry (UPLC/TOF-MS); Multivariate analysis; Myocardial infarction; Lipid biomarkers; LIPID-PEROXIDATION; ARACHIDONIC-ACID; HEART-DISEASE; METABONOMICS; REPERFUSION; ISCHEMIA; URINE; MS;

D O I：

10.1016/j.talanta.2009.03.045

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOF-MS)-based metabolomic approach was developed to characterize the metabolic profile associated with isoproterenol (ISO)-induced myocardial infarction (MI). Analysis of the serum samples revealed distinct changes in the biochemical patterns of ISO-induced rats. A multivariate statistical method, supervised partial least squares-discriminant analysis (PLS-DA), was then used for screening of potential biomarkers. As a result, 13 lipid biomarkers, including lysophosphatidylcholines (Lyso-PCs) and fatty acids were identified by the accurate mass measurement of TOF-MS. The relationship between abnormal lipid metabolism and the formation of MI were also studied. This work demonstrates the utility of UPLC/TOF-MS-based metabolic profiling combined with multivariate analysis as a powerful tool to further investigate the pathogenesis of cardiovascular diseases. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：254 / 259

页数：6

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