Effects of carbon dioxide/bicarbonate on induction of DNA single-strand breaks and formation of 8-nitroguanine, 8-oxoguanine and base-propenal mediated by peroxynitrite

被引：140

作者：

Yermilov, V ^{[1
]}

Yoshie, Y ^{[1
]}

Rubio, J ^{[1
]}

Ohshima, H ^{[1
]}

机构：

[1] INT AGCY RES CANC, UNIT ENDOGENOUS CANC RISK FACTORS, F-69372 LYON 08, FRANCE

来源：

FEBS LETTERS | 1996年 / 399卷 / 1-2期

基金：

日本学术振兴会;

关键词：

carbon dioxide; peroxynitrite; 8-nitroguanine; 8-oxoguanine; DNA strand break; thymine-propenal;

D O I：

10.1016/S0014-5793(96)01288-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbon dioxide has been reported to react with peroxynitrite (ONOO-), a strong oxidant and nitrating agent, to form an ONO2CO2- adduct, altering the reactivity characteristic of peroxynitrite. We found that bicarbonate (0-10 mM) caused a dose-dependent increase of up to 6-fold in the formation of 8-nitroguanine in calf-thymus DNA incubated with 0.1 mM peroxynitrite, whereas it produced no apparent effect on 8-oxoguanine formation. In contrast, bicarbonate inhibited peroxynitrite-induced strand breakage in plasmid pBR322 DNA and thymine-propenal formation from thymidine. We conclude that CO2/HCO3- reacts with peroxynitrite to form a potent nitrating agent, but also to inactivate hydroxyl-radical-like activity of peroxynitrous acid.

引用

页码：67 / 70

页数：4

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