Characterization and phylogenetic analysis of biodemulsifier-producing bacteria

被引：38

作者：

Huang, Xiang-Feng ^{[1
]}

Guan, Wei ^{[1
]}

Liu, Jia ^{[1
]}

Lu, Li-Jun ^{[1
]}

Xu, Jing-Cheng ^{[1
]}

Zhou, Qi ^{[1
]}

机构：

[1] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 20092, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 01期

关键词：

Biodemulsifier-producing bacteria; Demulsification; Phylogenetic analysis; Biosurfactant; IN-WATER EMULSIONS; DE-EMULSIFICATION; NOCARDIA-AMARAE; BACILLUS-SUBTILIS; GENUS NOCARDIA; OIL-EMULSIONS; RHODOCOCCUS; BIOSURFACTANT; IDENTIFICATION; CULTURE;

D O I：

10.1016/j.biortech.2009.07.086

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Based on demulsification performance, twenty biodemulsifier-producing strains were isolated from various environmental sources. Five of them achieved nearly or over 90% of emulsion breaking ratio within 24 h. With the aid of biochemical and physiological tests and 16S rDNA analysis, these isolates were classified into eleven genera, in which six genera (Brevibacillus sp., Dietzia sp., Ochrobactrum sp., Pusillimonas sp., Sphingopyxis sp. and Achromobacter sp.) were firstly reported as demulsifying strains. Moreover, with data in this study and other literatures, a phylogenic tree was constructed, showing a rich diversity of demulsifying bacteria. Half of these bacteria belong to Actinobacterale order, which is famous for hydrocarbon degradation and biosurfactant biosynthesis. However, some strains in the same genera differed remarkably in demulsifying capability, surface properties and biochemical and physiological characteristics. This implied the biosynthesis and composition of biodemulsifier were more complicated than expected. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：317 / 323

页数：7

共 35 条

[21]

Ma Ting, 2006, Huanjing Kexue, V27, P1191

[22] Evaluation of a mixed bacterial culture for de-emulsification of water-in-petroleum oil emulsions [J].

Nadarajah, N ;

Singh, A ;

Ward, OP .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2002, 18 (05) :435-440

[23]

NISHIMAKI F, 1999, FREEPATENTSONLINE PA

[24] Candida and Torulopsis: A blinded evaluation of use of pseudohypha formation as basis for identification of medically important yeasts [J].

Odds, FC ;

Rinaldi, MG ;

Cooper, CR ;

Fothergill, A ;

Pasarell, L ;

McGinnis, MR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (01) :313-316

[25] Demulsification of oil-in-water emulsions by aerial spores of a Streptomyces sp. [J].

Park, SH ;

Lee, JH ;

Ko, SH ;

Lee, DS ;

Lee, HK .

BIOTECHNOLOGY LETTERS, 2000, 22 (17) :1389-1395

[26] PHYLOGENETIC ANALYSIS OF THE GENERA RHODOCOCCUS AND NOCARDIA AND EVIDENCE FOR THE EVOLUTIONARY ORIGIN OF THE GENUS NOCARDIA FROM WITHIN THE RADIATION OF RHODOCOCCUS SPECIES [J].

RAINEY, FA ;

BURGHARDT, J ;

KROPPENSTEDT, RM ;

KLATTE, S ;

STACKEBRANDT, E .

MICROBIOLOGY-UK, 1995, 141 :523-528

[27]

RUIMY R, 1994, FEMS MICROBIOL LETT, V123, P261, DOI 10.1016/0378-1097(94)00382-3

[28] Characterization of hydrocarbonoclastic bacterial communities from mangrove sediments in Guanabara Bay, Brazil [J].

S Brito, Elcia Margareth ;

Guyoneaud, Remy ;

Goni-Urriza, Marisol ;

Ranchou-Peyruse, Antony ;

Verbaere, Arnaud ;

Crapez, Miriam A. C. ;

Wasserman, Julio Cesar A. ;

Duran, Robert .

RESEARCH IN MICROBIOLOGY, 2006, 157 (08) :752-762

[29] BACTERIA-INDUCED DE-EMULSIFICATION OF WATER-IN-OIL PETROLEUM EMULSIONS [J].

STEWART, AL ;

GRAY, NCC ;

CAIRNS, WL ;

KOSARIC, N .

BIOTECHNOLOGY LETTERS, 1983, 5 (11) :725-730

[30] Exopolysaccharides produced by Gordonia alkanivorans enhance bacterial degradation activity for diesel [J].

Ta-Chen, Lin ;

Chang, Jo-Shu ;

Young, Chiu-Chung .

BIOTECHNOLOGY LETTERS, 2008, 30 (07) :1201-1206

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