Differential gene expression profiles in the hippocampus of senescence-accelerated mouse

被引:34
作者
Cheng, Xiao-Rui
Zhou, Wen-Xia [1 ]
Zhang, Yong-Xiang
Zhou, Dong-Sheng
Yang, Rui-Fu
Chen, Ling-Feng
机构
[1] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
[2] Beijing Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
[3] Chinese Acad Sci, Key Lab Photosynth & Environm Mol Physiol, Inst Bot, Beijing 100093, Peoples R China
基金
中国国家自然科学基金;
关键词
senescence-accelerated mouse; Alzheimer's disease; cDNA microarray;
D O I
10.1016/j.neurobiolaging.2006.02.004
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
The senescence-accelerated mouse (SAM) is an animal model for studying senescence and age-associated disorders due to its inherited aging phenotype. The SAM/prone8 (SAMP8) is a useful animal model to investigate the fundamental mechanisms involved in age-related learning and memory deficits that may have relevance to age-associated AD, while SAM/resistant1 (SAMR1) shows normal. To identify genes rendering the cognitive deterioration with aging, the subtractive cDNA libraries containing 1924 clones with the positive ratio of 96.18% were generated and the microarray containing 3136 cDNA was prepared. The results of screening libraries by the microarray showed that of all 91 differentially expressed genes, 50 were over-expressed and 41 were low-expressed in SAMP8. Some of the identified genes were confirmed. by the real time quantitative RT-PCR. These results indicated the profiles of gene expression in the hippocampus of SAMP8 and SAMR1 were significantly different, which may play important roles in the age-related cognitive deficit in SAMP8, suggesting those genes related to the cognitive deficient or pathology change in the brain of SAMP8 may be potential gene targets for Alzheimer's disease therapy. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:497 / 506
页数:10
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