Chemical-biogeographic survey of secondary metabolism in soil

被引：104

作者：

Charlop-Powers, Zachary ^{[1
]}

Owen, Jeremy G. ^{[1
]}

Reddy, Boojala Vijay B. ^{[1
]}

Ternei, Melinda A. ^{[1
]}

Brady, Sean F. ^{[1
]}

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 10期

基金：

美国国家卫生研究院;

关键词：

metagenomics; antibiotics; bioprospecting; eDNA; NONRIBOSOMAL PEPTIDE SYNTHETASES; POLYKETIDE SYNTHASE GENES; COMPLETE GENOME SEQUENCE; BIOSYNTHESIS; DIVERSITY; CLUSTERS;

D O I：

10.1073/pnas.1318021111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study, we compare biosynthetic gene richness and diversity of 96 soil microbiomes from diverse environments found throughout the southwestern and northeastern regions of the United States. The 454-pyroseqencing of nonribosomal peptide adenylation (AD) and polyketide ketosynthase (KS) domain fragments amplified from these microbiomes provide a means to evaluate the variation of secondary metabolite biosynthetic diversity in different soil environments. Through soil composition and AD-and KS-amplicon richness analysis, we identify soil types with elevated biosynthetic potential. In general, arid soils show the richest observed biosynthetic diversity, whereas brackish sediments and pine forest soils show the least. By mapping individual environmental amplicon sequences to sequences derived from functionally characterized biosynthetic gene clusters, we identified conserved soil type-specific secondary metabolome enrichment patterns despite significant sample-to-sample sequence variation. These data are used to create chemical biogeographic distribution maps for biomedically valuable families of natural products in the environment that should prove useful for directing the discovery of bioactive natural products in the future.

引用

页码：3757 / 3762

页数：6

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