Structural dynamics and processing of nucleic acids revealed by single-molecule spectroscopy

被引：80

作者：

Ha, TJ ^{[1
]}

机构：

[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[2] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

来源：

BIOCHEMISTRY | 2004年 / 43卷 / 14期

关键词：

D O I：

10.1021/bi049973s

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule fluorescence spectroscopy is a powerful method to observe real time movements of individual biological molecules while they are functioning without the need for synchronization. Dynamic characteristics of nucleic acids can now be easily and reliably studied, and new applications are emerging in which their recognition and processing by proteins and enzymes are being understood with unprecedented detail. The most recent examples are discussed, including the hairpin ribozyme, Holliday junction, G-quadruplex, Rep helicase, reverse transcriptase, and combination with mechanical manipulation.

引用

页码：4055 / 4063

页数：9

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