Establishment and characterization of a new mantle cell lymphoma cell line, Mino

被引:43
作者
Lai, R
McDonnell, TJ
O'Connor, SL
Medeiros, LJ
Oudat, R
Keating, M
Morgan, MB
Curiel, TJ
Ford, RJ
机构
[1] Univ Texas, MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX 77030 USA
[2] Univ Texas, MD Anderson Canc Ctr, Dept Mol Pathol, Houston, TX 77030 USA
[3] Univ Texas, MD Anderson Canc Ctr, Dept Leukemia, Houston, TX 77030 USA
[4] Baylor Inst Immunol Res, Dallas, TX USA
关键词
mantle cell lymphoma; cell line; Mino; cyclin D1; p53;
D O I
10.1016/S0145-2126(02)00013-9
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Mantle cell lymphoma (MCL) is a distinct type of B-cell non-Hodgkin's lymphoma characterized by cyclin D1 overexpression and the cytogenetic abnormality, the t(11; 14)(q13;q32). MCL cell lines have been difficult to establish and in vitro studies of these neoplasms are scarce. We describe the establishment and characteristics of a new MCL cell line, Mino. The cells are large, growing singly and in small clumps in vitro. By flow cytometry, the immunophenotype was compatible with MCL (i.e. CD5(+)CD20(+)CD23(-)FMC7(+)). Conventional cytogenetics showed hyperdiploidy with multiple complex karyotypic abnormalities, but no evidence of the t(I 1; 14), proven to be present only by fluorescence in situ hybridization and polymerase chain reaction (PCR) methods. Western blots showed expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein was predominantly phosphorylated. There was expression of tumor suppressor gene products including p53, p16(INK4a), and p21(WAF1). Sequencing of the TP53 gene revealed a mutation (codon 147(valine-->glycine)) in exon 5. Epstein Barr virus was absent. In summary, Mino is a new MCL cell line that may be useful to study the pathogenesis of MCL. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:849 / 855
页数:7
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