Experimental studies on the growth and usnic acid production in "lichen" Usnea ghattensis in vitro

被引：20

作者：

Behera, B. C. ^{[1
]}

Verma, Neeraj ^{[1
]}

Sonone, Anjali ^{[1
]}

Makhija, Urmila ^{[1
]}

机构：

[1] Agharkar Res Inst, Plant Sci Div, Pune 411004, Maharashtra, India

来源：

MICROBIOLOGICAL RESEARCH | 2006年 / 161卷 / 03期

关键词：

lichen; culture condition; usnic acid;

D O I：

10.1016/j.micres.2005.08.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The study was aimed to optimize the culture conditions for the production of usnic acid in the cultured cell aggregates composed of symbionts in Lichen Usnea ghattensis in vitro. The cultured Lichen tissue composed of symbionts appeared after about 2-3 weeks of inoculation in water-agar and malt-yeast extract (MYE) media and shown the production of usnic acid after 2-3 months of inoculation. However, the growth of symbionts was strongly affected by different culture conditions. The addition of excess carbon and nitrogen sources in the media has significantly enhanced the growth as well as usnic acid content. The cultured symbionts in MYE medium having 4% sucrose, 4% polyethyl glycol. (PEG) gave 7.63 g dry biomass with 3.9 mu g usnic acid/g dry biomass. In water-agar medium having 4% sucrose and 4% PEG gave 3.08 g dry biomass with 1.11 mu g usnic acid/g dry biomass. The positive effects of medium on the growth of symbionts and the production of usnic acid are seemed to be due to nutritional factors. (c) 2005 Elsevier GmbH. All rights reserved.

引用

页码：232 / 237

页数：6

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